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URL: http://www.addgene.org/33349
Proper Citation: RRID:Addgene_33349
Bacterial Resistance: Ampicillin
Defining Citation: PMID:14702347
Vector Backbone Description: Backbone Marker:5500; Vector Backbone:pRc/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The polylinker region of the pRc/CMV eukaryotic expression vector (Invitrogen) was cut with NdeI and HindIII, and the following annealed oligonucleotide was inserted with a hemagglutinin epitope (YPYDVPDYASL) and NdeI-HindIII cloning site was made using the following pair of complementary oligonucleotides: 5′-AGCTCCACCATGGCGTATCCCTACGACGTGCCCGATTATGCCCATATGTGATGAAGCTT-3′ (underlined bases are restriction sites for NcoI, NdeI, and HindIII) and 5′-CTAGAAGCTTCATCACATATGGGCATAATCGGGCACGTCGTAGGGATACGCCATGGTGG-3′ (underlined bases are restriction site for XbaI).
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Source: Addgene