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URL: http://www.addgene.org/19978
Proper Citation: RRID:Addgene_19978
Insert Name: TEV protease
Organism: tobacco etch virus
Bacterial Resistance: Kanamycin
Defining Citation: PMID:10873547
Vector Backbone Description: Backbone Marker:Herman Bujard; Backbone Size:4300; Vector Backbone:pZS*24-MCS1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: This TEV protease expression vector is intended to be used for controlled intracellular processing of fusion proteins (TEV protease substrates) in E. coli. It is supplied in BL21-Pro cells so that transcription of TEV protease can be regulated by anhydrotetracycline. Replicon: pSC101 Promoter: Synthetic ªPL/tetO Inducer: anhydrotetracycline (100 ºg/liter) Notes: Expression of the TEV protease gene on pKM586 will be constitutive unless the host strain produces Tet repressor. Regulated expression can be achieved in DH5alphaPro or BL21Pro cells (Clontech). For best results, perform intracellular processing experiments at 30 °C.
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Source: Addgene
Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
