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URL: http://www.addgene.org/19750
Proper Citation: RRID:Addgene_19750
Insert Name: oligo targeting Firefly luciferase
Bacterial Resistance: Ampicillin
Defining Citation: PMID:18779577
Vector Backbone Description: Backbone Marker:MSCV2.2 with WPRE cloned into ClaI site is the base vector; Backbone Size:7518; Vector Backbone:MSCV P2Gm; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Comments: MSCV-based entry vector for cloning and validating miR30-based RNAi constructs. The vector expresses puromycin-resistance and GFP, separated by the FMDV 2A peptide. This is considered the "empty" vector and can be used for cloning. It contains a hairpin targeting Firefly luciferase. See FLIP vector protocols (PDF from this page) for more information. There are minor discrepancies between the depositor's sequence and Addgene's sequencing results. The mismatches are either coding joints or a sequencing difference between the actual and the published miR30 vector sequence.
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Source: Addgene
Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
