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URL: http://www.addgene.org/1954
Proper Citation: RRID:Addgene_1954
Insert Name: BAF155
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:10078207
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: We constructed a composite full-length cDNA for hSWI3 (BAF155) using a 2.4 kb partial human cDNA that was isolated as described previously and a 1.8 kb RT-PCR-amplified fragment obtained from human liver poly(A) RNA. We consistently isolated the partial hSWI3 cDNA (2.4 kb) from several libraries. It appears that an adenine-rich stretch at the 3' end of these cDNAs hybridizes with the oligo(dt) primers used to create the first strand of the cDNA. An oligo that anneals in the sense direction to the partial cDNA, and an oligo that anneals to the 3' UTR of the highly related hSWI3 clone described previously, BAF155, were used to generate the missing coding sequences. The amplified fragment was inserted into the BamHI and SpeI sites of pBS-5'hSWI3, which contains the partial hSWI3 cDNA. The protein encoded by this complete ORF is highly related to BAF155, except for a few positions scattered throughout the protein. Most of these differences are substitutions of BAF155 sequences with the corresponding sequences of BAF170. To generate pFastBac1-BAF155, an XhoI
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Source: Addgene
Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
