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URL: http://www.addgene.org/117510
Proper Citation: RRID:Addgene_117510
Insert Name: BsaI:GGAG:pAG:AATG:BsaI
Organism: Arabidopsis thaliana
Bacterial Resistance: Spectinomycin
Defining Citation: PMID:30625150
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41295; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Comments: pICSL12022 carries GGAG-TACT golden gate ends. It has to be cloned with a 5’UTR plasmid, TACT-AATG, when assembled with a CDS and terminator into Level 1. For instance pICH41402 (plasmid 50285 on Addgene) can be used. This assembly of promoter / 5’UTR / CDS / terminator can be done in a single reaction. Cloned by Laurence Tomlinson . Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint.
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Source: Addgene
Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
