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URL: http://www.addgene.org/11724
Proper Citation: RRID:Addgene_11724
Insert Name: b-lactamase
Organism: E. coli
Bacterial Resistance: Kanamycin
Defining Citation: PMID:11602576
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2642; Vector Backbone:modified pGPS3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Positive control for mutagenesis experiments: reversion of the stop codon results in ampicillin resistance. The pGPS3 has a deletion from BglI to BglII that destroys the ampicillin resistance gene. The ampicillin resistance gene containing the stop codon was then cloned in between AflIII and KpnI. Please also see pGFPuv from BD Biosciences as another control for forward mutation assays. Catalog # 6079-1 http://www.clontech.com/US/Products/Fluorescent_Proteins_and_Reporters/Fluorescent_Proteins_by_Name/GFP_GFPuv_Fluorescent_Proteins?sitex=10020:22372:US .
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Source: Addgene
Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
