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URL: http://www.addgene.org/111702
Proper Citation: RRID:Addgene_111702
Insert Name: APEX2
Organism: Other
Bacterial Resistance: Kanamycin
Defining Citation: PMID:29708735
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: This plasmid is for IPTG-inducible expression of APEX2 in the E. coli cytoplasm. The APEX gene was subcloned from pcDNA3-mito-APEX and subsequently mutated by site-directed mutagenesis to generate the optimized variant APEX2. See also references: Rhee HW, Zou P, Udeshi ND, Martell JD, Mootha VK, Carr SA, Ting AY. Proteomic mapping of mitochondria in living cells via spatially restricted enzymatic tagging. Science. 2013;339(6125):1328-31. PMCID: 3916822. Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY. Directed evolution of APEX2 for electron microscopy and proximity labeling. Nat Methods. 2015;12(1):51-4. PMCID: 4296904
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Source: Addgene
Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
