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URL: http://www.addgene.org/110274
Proper Citation: RRID:Addgene_110274
Insert Name: Elongin BC
Organism: Homo sapiens
Bacterial Resistance: Chloramphenicol
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYC-Duet1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Full length Elongin B was cloned into MCS1 NcoI site of pACYC-Duet1. Elongin C (a.a. 17-112) was cloned in 2nd MCS with stop codon before any 3’ vector S tag. Therefore, these inserts produce untagged proteins. Note: ATG from NdeI site precedes internal start Met17 of Elongin C giving start sequence MMYVK... 2C9W: https://www.thesgc.org/structures/2C9W.
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Source: Addgene
Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
