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URL: http://www.addgene.org/10957
Proper Citation: RRID:Addgene_10957
Insert Name: MDR1
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:2898143
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pHa; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: Full-length cDNA, has the wild-type glycine at position 185 on P-glycoprotein (see GenBank ID M14758.1 for the sequence variations at nucleotides 382, 964, 978, 979, 1660 and 2065 that are present in this plasmid) The "Ha" in pHaMDRwt stand for the Harvey sarcoma virus. Vector can confer the complete multidrug-resistance phenotype to various drug sensitive cell lines. Figure legend for author's map: Structure of retroviral vector carrying MDR] cDNA. sites: B, BamHI; E, EcoRI; H, HindIII; S, Stu I; X, Xho I. bp, Base pairs; amp, ampicillin resistance gene.
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Source: Addgene