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Plasmid Name
RRID:Addgene_10957 RRID Copied  
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RRID:Addgene_10957
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Plasmid Information

URL: http://www.addgene.org/10957

Proper Citation: RRID:Addgene_10957

Insert Name: MDR1

Organism: Homo sapiens

Bacterial Resistance: Ampicillin

Defining Citation: PMID:2898143

Vector Backbone Description: Backbone Size:11000; Vector Backbone:pHa; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin

Comments: Full-length cDNA, has the wild-type glycine at position 185 on P-glycoprotein (see GenBank ID M14758.1 for the sequence variations at nucleotides 382, 964, 978, 979, 1660 and 2065 that are present in this plasmid) The "Ha" in pHaMDRwt stand for the Harvey sarcoma virus. Vector can confer the complete multidrug-resistance phenotype to various drug sensitive cell lines. Figure legend for author's map: Structure of retroviral vector carrying MDR] cDNA. sites: B, BamHI; E, EcoRI; H, HindIII; S, Stu I; X, Xho I. bp, Base pairs; amp, ampicillin resistance gene.

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Data and Source Information

Source: Addgene