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URL: http://www.addgene.org/109301
Proper Citation: RRID:Addgene_109301
Insert Name: Bxb1 attB site
Organism: Synthetic
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:30538179
Vector Backbone Description: Backbone Size:3082; Vector Backbone:pKM464; Vector Types:ORBIT integrating plasmid; Bacterial Resistance:Chloramphenicol
Comments: ORBIT plasmid for creating deletion of mycobacterial target genes, using zeocin as a resistance marker. Strain should be grown in 20 ug/ml chloramphenicol for isolation of plasmid. Yields with Qiagen miniprep kits are typically in the range from 60-90 ug/ml from 5 ml culture.
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No rating or validation information has been found for pKM496 (deletions) Zeo.
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Source: Addgene
Data set from Kravitz, Owen and Kretizer's 2012 paper "Optogenetic identification of striatal projection neuron subtypes during in vivo recordings."
