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The Human Islet Research Network (HIRN) helps to organize and support collaborative research related to the loss of functional beta cell mass in Type 1 Diabetes. The protocols view is a list of protocols used by HIRN researchers. The "Name" column links to the HIRN individual protocol pages, and the "Protocols.io" column links to protocols.io, where detailed information may be found.

(last updated: Mar 5, 2022)

Information Protocols

52 Results - per page

NameCategoryProtocols.ioReference
Human Foxp3 Staining protocolsample preparation for assayYes
Protocols for Blood Collection from Subjects with New Onset Type 1 Diabetessample preparation for assayYes
Human Islet Proteomics SOP for HIRNsample preparation for assayYes
Surface Staining for Flow Cytometrysample preparation for assayYes
Generation of engraftment competent hematopoietic progenitors from human iPS cellssample preparation for assayYesPMID:28481220
Human Peripheral Blood Mononuclear Cell (PBMC) Isolation Procedureisolation of PBMCsYes
The production of monocytes and macrophages from human iPSCsYesPMID:29437254
Serum-free, stromal cell-free differentiation protocol for generating high percentages of hematopoietic cells from a human iPSC lineYes
Live Cell Monitoring and Enrichment of Stem Cell‐Derived β Cells Using Intracellular Zinc Content as a Population MarkerYesPMID:31756031
Human Islet Cell Culture ProtocolYes
Purification of Live Stem-Cell-Derived Islet Lineage IntermediatesYesPMID:32521122
Pancreatic Beta Cell Differentiation From Human Pluripotent Stem CellsYesPMID:30192441
Generation of Immunodeficient Mice Bearing Human Immune Systems by the Engraftment of Hematopoietic Stem CellsYesPMID:27150084
Using pancreas tissue slices for in situ studies of islet of Langerhans and acinar cell biologyYes
Using Pancreas Tissue Slices for the Study of Islet PhysiologyYes
Generation of functional human thymic cells from induced pluripotent stem cellsYesPMID:34331993
Umbilical cord-derived mesenchymal stem cells for COVID-19 patients with acute respiratory distress syndrome (ARDS)Yes
POSEIDON study: a pilot, safety and feasibility trial of high­-dose omega­3 fatty acids and high-­dose cholecalciferol supplementation in type 1 diabetesYes
Cellular Composition and Fractional β-Cell Viability Assay Protocol at the Diabetes Research Institute – University of MiamiYes
Human Vertebral Body Marrow Processing: Standard Operating Procedure from the cGMP Human Cell Processing Facility of the Cell Transplant Center and Diabetes Research Institute at the University of MiamiYes
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