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The Human Islet Research Network (HIRN) helps to organize and support collaborative research related to the loss of functional beta cell mass in Type 1 Diabetes. The constructs view is a list of constructs used by HIRN researchers. The "Name" column links to the HIRN individual construct pages.
(last updated: Mar 7, 2022)
Molecule Physical Resource or Software Tool| Name | Description | Insert Name | Vector | Vector Type | Vector Backbone Name | Vendor | |
|---|---|---|---|---|---|---|---|
| pMDLg.RRE (46b) | Contains Gag & Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding Rev | HIV-1 GAG/POL | viral vectors - pMD | viral vectors | pMD | Addgene plasmid | 12251 |
| pLV[Exp]-neo-EF1a-CD19-T2A-eGFP | CD19 is a type of white blood cell found on the surface of B-cells; lentivial expression plasmid (3rd generation) | CD19-T2A-eGFP_2451bp | viral vectors | VectorBuilder | VB161129-1084utf | ||
| pCDH-ef1a-INS-DDK-eGFP-PuroR | insulin gene (decreases blood glucose concentration) cloned into mamalian expression plasmid with GFP and puro resistance | System Biosciences | |||||
| pCDH-cyto-BirA (Myc-BirA) | cloned the Myc-BirA out of the retrovirus vector pOP-Av and into the lentivirus vector pCDH-EF1α-T2A-puro | System Biosciences | |||||
| pCDH-ef1a-MLANA-DDK-eGFP-PuroR | MLANA gene cloned into mamalian expression plasmid with GFP and puro resistance for overexpression | System Biosciences | |||||
| pCDH-cyto-Myc BirA | single promoter BirA control plasmid for PTPN22 in the lentiviral expression vector pCDH with the EF1a promoter | System Biosciences | |||||
| pMD2-VSVG ENDOfree | VSVG expressing envelope plasmid, also called pMD2.G | VSV G | plasmids - pMD2.G | plasmids | pMD2.G | Addgene plasmid | 11259 |
| pRSV.REVB | 3rd generation transfer plasmid | viral vectors | Addgene plasmid | 12253 | |||
| pOP-BirA-PTPN22(W)-eGFP | retrovirus plasmid used for PTPN22 BioID containing E. coli biotin ligase BirA with the mutation in PTPN22 at AA 620 (T->W) |
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The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant 2U24DK097771-09.
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