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Trinity Biobank

The Trinity Biobank was established in 2005 to serve the needs of researchers in the area of genetic epidemiology, population genetics and pharmacogenomics. Its services are available to researchers not only in Trinity College but to other institutions at home and abroad. We provide an automated DNA extraction service purifying large volumes blood (up to 10mL whole blood) and tissue DNA for archival and other purposes. In addition it makes available purified DNA and associated GWAS data from 2000 healthy donors for research use. A key requirement for reliable downstream use of DNA is purity and strand size. The quality of DNA in blood and tissue deteriorates upon storage without purification even at -80 degrees C. We ensure rapid turnaround of biological samples through automated extraction using the Qiagen Autopure system based on optimized 'salting out' chemistry. The purified DNA sample may then be stored safely at -20 degrees C without deterioration thus freeing up valuable -80 degree C freezer space and the associated capital and maintenance cost as well as security and lab space provision. Automated DNA extraction is particularly suitable for high-throughput sample processing called for in epidemiological studies or simply for clearing sample inventory backlogs. The Trinity Biobank distributes control DNA to researchers as part of its remit to enhance the level of research activity and to synergize molecular medicine research nationally and internationally. The buffy coat collection has been made possible with the cooperation of the Irish Blood Transfusion Service (IBTS). An important requirement to access the collection is that the use of the samples relates only to ethically-approved research and to specifically-nominated research projects. The DNA collection consists of high quality human genomic DNA. Each of the available 2,000 samples is from a single individual and each sample comes with the age and gender data of the donor. The buffy coat sample is derived from the total white cell compliment (50mL buffy coat) of a blood donation (c 400mL). We will endeavor to fulfill samples number requests based on age and gender as best as possible. This collection has also been genotyped using the Affymetrix Genome-Wide Human SNP Array 6.0, featuring 1.8 million genetic markers, including more than 906,600 single nucleotide polymorphisms (SNPs) and more than 946,000 probes for the detection of copy number variation (CNV). The DNA comes available as a 100ng/uL in 100uL of TE Buffer, ie in 10ug amounts in a separate screw-cap ampoule. The ampoules are shipped in 100-tube boxes (Sarstedt). Corresponding plasma (ACD) is also available on request. Genotype data is supplied in PLINK binary PED files format (http://pngu.mgh.harvard.edu/~purcell/plink/ ).

URL: http://www.tcd.ie/IMM/trinity-biobank/index.php

Resource ID: nlx_25068     Resource Type: Resource     Version: Latest Version

Keywords

dna, blood, tissue, buffy coat, cell, packed cell, cultured cell, buccal cell, whole blood, bodily fluid, fresh, frozen, healthy, normal, genetic epidemiology, population genetics, pharmacogenomics, gene, genetics, genome-wide association study, genome-wide association study data, genotype, dna extraction, non-mammalian, bacteria, gram-positive bacteria, gram-negative bacteria

Additional Resource Types

biomaterial supply resource, material service resource, material storage repository, data set

Availability

Public: Its services are available to researchers not only in Trinity College but to other institutions at home and abroad. The Trinity Biobank distributes control DNA to researchers as part of its remit to enhance the level of research activity and to synergize molecular medicine research nationally and internationally. It makes available purified DNA and associated GWAS data from 2000 healthy donors for research use.

Related Application

Research

Processing

Fresh, Frozen

Abbreviation

Trinity Biobank

Comment

Sample type: DNA, Blood, Tissue, Buffy coat, Cell, Packed cell, Cultured cell, Buccal cell, Whole blood, Bodily fluid

Parent Organization

Species

human

Supercategory

Resource

Curation Status

uncurated

Listed By

One Mind Biospecimen Bank Listing, European Biobank

Old URLs

http://www.tcd.ie/IMM/research_biobank.php

Related Disease

Healthy, Normal

Resource Status

Last checked up;

Original Submitter

Anonymous

Version Status

Curated

Submitted On

12:00am August 17, 2011

Originated From

SciCrunch

Changes from Previous Version

First Version

Version 1

Created 4 years ago by Anonymous