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Mouse Brain Architecture Project

An atlas project whose goal is to enerate brainwide maps of inter-regional neural connectivity that specify the inputs and outputs of every brain region, at a "mesoscopic" level of analysis. A 3D injection viewer is used to view the mouse brain. To determine the outputs of a brain region, anterograde tracers are used which are taken up by neurons locally ("the input"), then transported actively down the axons to the "output regions." The whole brain is then sliced thinly, and each slice is digitally imaged. These 2-D images are reconstructed in 3D. The majority of the resulting 3-D brain image is unlabeled. Only the injected region and its output regions have tracer in them, allowing for identification of this small fraction of the connectivity map. This procedure is repeated identically, to account for individual variability. To determine the inputs to the same brain region as above, a retrograde tracer is injected in the same stereotaxic location ("the input"), and the process is repeated. In order to accumulate data from different mice (each of whom has a slightly different brain shape and size), 3-D spatial normalization is performed using registration algorithms. These gigapixel images of whole-brain sections can be zoomed to show individual neurons and their processes, providing a "virtual microscope." Each sampled brain is represented in about 500 images, each image showing an optical section through a 20 micron-thick slice of brain tissue. A multi-resolution viewer permits users to journey through each brain, following the pathways taken through three-dimensional brain space by tracer-labeled neuronal pathways. A key point is that at the mid-range "mesoscopic" scale, the team expects to assemble a picture of connections that are stereotypical and probably genetically determined in a species-specific manner. By dividing the volume of a hemisphere of the mouse brain into 250 equidistant, predefined grid-points, and administering four different kinds of tracer injections at each grid point -- in different animals of the same sex and age a complete wiring diagram that will be stitched together in "shotgun" fashion from the full dataset.

URL: http://www.brainarchitecture.org/mouse-home

Resource ID: nlx_146201     Resource Type: Resource     Version: Latest Version

Keywords

atlas, brain, brain architecture, connectivity, mouse brain architecture, neuroanatomy

Additional Resource Types

Data or Information Resource

Alternate URLs

http://www.brainarchitecture.org

Species

mouse

License

Creative Commons Attribution-ShareAlike 3.0 Unported License: Users are free to share (copy and redistribute) and adapt (remix, transform, and build upon) the material in any medium or format as long as users attribute the Mouse Brain Architecture Project (MBA) and provide a link to the MBA and the CC licenses provided. If users adapt the MBA material, they must distribute their contributions under the same license as the MBA original.

Related To

Resource: Brain Architecture Project

Synonyms

Mouse Brain Architecture, MBA Project

Parent Organization

Funding Information

Office of the Director, NIH, RC1MH088659, R01MH087988

Availability

Fully accessible to the neuroscience community as well as interested members of the general public, Acknowledgement requested

Old URLs

http://www.brainarchitecture.org/mouse/about

Supercategory

Resource

Original Submitter

Anonymous

Version Status

Curated

Submitted On

12:00am June 11, 2012

Originated From

SciCrunch

Changes from Previous Version

  • Description was changed

Version 3

Created 3 weeks ago by Kristen Jensen

Version 2

Created 3 weeks ago by Kristen Jensen

Version 1

Created 3 years ago by Anonymous