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Honey Bee Brain EST Project

A database integrating data from the bee brain EST sequencing project with data from sequencing and gene research projects from other organisms, primarily the fruit fly Drosophila melanogaster. The goal of Bee-ESTdb is to provide updated information on the genes of the honey bee, currently using annotation primarily from flies to suggest cellular roles, biological functions, and evolutionary relationships. The site allows searches by sequence ID, EST annotations, Gene Ontology terms, Contig ID and using BLAST. Very nice resource for those interested in comparative genomics of brain. A normalized unidirectional cDNA library was made in the laboratory of Prof. Bento Soares, University of Iowa. The library was subsequently subtracted. Over 20,000 cDNA clones were partially sequenced from the normalized and subtracted libraries at the Keck Center, resulting in 15,311 vector-trimmed, high-quality, sequences with an average read length of 494 bp. and average base-quality of 41. These sequences were assembled into 8966 putatively unique sequences, which were tested for similarity to sequences in the public databases with a variety of BLAST searches. The Clemson University Genomics Institute is the distributor of these public domain cDNA clones. For information on how to purchase an individual clone or the entire collection, please contact www.genome.clemson.edu/orders/ or generobi (at) life.uiuc.edu.

URL: http://titan.biotec.uiuc.edu/bee/honeybee_project.htm

Resource ID: nif-0000-00118     Resource Type: Resource     Version: Latest Version

Keywords

expressed sequence tag, brain, behavior, cdna, blast, gene, annotation, microarray, gene expression, comparative genomics, cdna clone, resource:genbank

Funding Information

NSF, University of Illinois Critical Research Initiatives Program, Burroughs Wellcome Fund,

Additional Resource Types

database, biomaterial supply resource

Availability

Free

Species

honeybee

Related To

Resource:One Mind Biospecimen Bank Listing, Resource:GO

Abbreviation

Bee-ESTdb

Parent Organization

Supercategory

Resource

Synonyms

Honeybee EST Project

Comment

Sample type: cDNA clone,

Listed By

One Mind Biospecimen Bank Listing, Biobank

Related Application

Research

Original Submitter

Anonymous

Version Status

Pending

Submitted On

12:00am September 21, 2010

Originated From

SciCrunch

Changes from Previous Version

  • Description was changed
  • Related To was changed

Version 2

Created 2 days ago by Christie Wang

Version 1

Created 5 years ago by Anonymous

Annotated expressed sequence tags and cDNA microarrays for studies of brain and behavior in the honey bee.

  • Whitfield CW
  • Genome Res.
  • 2002 4

To accelerate the molecular analysis of behavior in the honey bee (Apis mellifera), we created expressed sequence tag (EST) and cDNA microarray resources for the bee brain. Over 20,000 cDNA clones were partially sequenced from a normalized (and subsequently subtracted) library generated from adult A. mellifera brains. These sequences were processed to identify 15,311 high-quality ESTs representing 8912 putative transcripts. Putative transcripts were functionally annotated (using the Gene Ontology classification system) based on matching gene sequences in Drosophila melanogaster. The brain ESTs represent a broad range of molecular functions and biological processes, with neurobiological classifications particularly well represented. Roughly half of Drosophila genes currently implicated in synaptic transmission and/or behavior are represented in the Apis EST set. Of Apis sequences with open reading frames of at least 450 bp, 24% are highly diverged with no matches to known protein sequences. Additionally, over 100 Apis transcript sequences conserved with other organisms appear to have been lost from the Drosophila genome. DNA microarrays were fabricated with over 7000 EST cDNA clones putatively representing different transcripts. Using probe derived from single bee brain mRNA, microarrays detected gene expression for 90% of Apis cDNAs two standard deviations greater than exogenous control cDNAs. [The sequence data described in this paper have been submitted to Genbank data library under accession nos. BI502708-BI517278. The sequences are also available at http://titan.biotec.uiuc.edu/bee/honeybee_project.htm.]