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3D surgical atlases of the murine head

3D interactive atlas of two mouse brains, 129S1/SvImJ and C57Bl/6J. The aim of this resource is to enhance comparative morphometric analyses and stereotactic surgical procedures in mice. These representations of the murine brain and skull, in conjunction with the resource's development of a new, more dynamic master coordinate system, provide improved accuracy with respect to targeting CNS structures during surgery compared with previous systems. The interactive three-dimensional nature of these atlases also provide users with stereotactic information necessary to perform accurate off-axis surgical procedures, as is commonly required for experiments such as in vivo micro-electroporation. In addition, three-dimensional analysis of the brain and skull shape in C57Bl, 129Sv, CD1, and additional murine strains, suggests that a stereotactic coordinate system based upon the lambda and rostral confluence of the sinuses at the sagittal midline, provides improved accuracy compared with the traditional lambdabregma landmark system. These findings demonstrate the utility of developing highly accurate and robust three-dimensional representations of the murine brain and skull, in which experimental outputs can be directly compared using a unified coordinate system.

URL: http://phm.utoronto.ca/~jeffh/surgical.htm

Resource ID: nif-0000-10249     Resource Type: Resource     Version: Latest Version

Keywords

129s1/svimj, anatomy, brain, c57bl/6j, central nervous system, digital atlas, imaging assay, in vivo, micro-electroporation, morphometric analysis, skull, sterotactic, transgenic, imaging

Synonyms

MRI / CT atlases

Funding Information

Canadian Institute for Health Research, Ontario Research Development Challenge, NARSAD Young Investigator award,

Additional Resource Types

atlas

Species

mouse

Supercategory

Resource

Parent Organization

Original Submitter

Anonymous

Version Status

Curated

Submitted On

12:00am October 18, 2010

Originated From

SciCrunch

Changes from Previous Version

First Version

Version 1

Created 5 years ago by Anonymous