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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ∆HHT1/∆HHT1; ∆HHT3/∆HHT3
hht1[∆::neo2]/hht1[∆::neo2];hht3[∆::neo2]/hht3[∆::neo2] (mpr1-1; pm-s, mp-r, ?)
Affected Genes: HHT1 + HHT3 (TTHERM_00570560, TTHERM_00016170)
Genomic Alteration: Micronucleus: Neo2 KO of HHT1 and HHT3
Source References: PMID:16908532
Notes: homozygous, Neo cassette knockout
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01879 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mrp1-1/mpr1-1 (twi1[3’neo3, FLAG-HAn]; pm-r, VII)
Affected Genes: TWI1 (TTHERM_01161040)
Genomic Alteration: Macronucleus: FLAG-HA tagged TWI1 with neo3 replaced all TWI1
Source References: PMID:12297043
Notes: All somatic TWI1 was replaced by FLAG-HA-TWI1 construct. CU428 background.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02609 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (CNA1/cna1[∆::neo2]; pm-r, VII)
Affected Genes: CNA1 (TTHERM_00146340)
Genomic Alteration: Macronucleus: neo2 into CNA1
Source References: PMID:16738316
Notes: CNA1 KO construct is transformed inWT mac, assorted to 500ug/ml pm resistance. Complete replacement not possible, gene is essential.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02693 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (Circular permutation of HHO1 E5 charge patch, impossible to define in correct nomenclature, see paper.; pm-r, VII)
Affected Genes: HHO1 (TTHERM_00823720)
Source References: PMID:11972045
Notes: Somatic transformation of CU428 with HHO1 construct E-C
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02294 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; hht3[∆::neo2]/hht3[∆::neo2] (mp-r, ?)
Affected Genes: HHT2 + HHT3 (TTHERM_00189180, TTHERM_00016170)
Genomic Alteration: Micronucleus: Neo2 KO of HHT3 and HHT2/HHF2 gene with its intergenic region
Source References: PMID:16908532
Notes: This is the non-star side of round I genomic exclusion. It should have the same mic genome as ∆H3.2/3.3 gl KO 1*strain
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01880 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (twi1[3’neo3, FLAG-HA]; FLAG-HA TWI1, pmr (neo3), VII)
Affected Genes: TWI1
Genomic Alteration: Macronucleus: FLAG-HA tagged TWI1 with neo3 replaced all TWI1
Source References: PMID:12297043
Notes: All somatic TWI1 was replaced by FLAG-HA-TWI1 construct. CU428 background.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02613 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1 (?HHT1/hht1[∆::neo2; hht2[3’neo2, S10A]; Somatic ∆HHT1 with neo cassette and HHT2S10A ( neo in 3 flank) cotransformant., VI)
Affected Genes: HHT1 (TTHERM_00570560), HHT2 (TTHERM_00189180)
Genomic Alteration: Macronucleus: KO of HHT1, HHT2 has mutation S10A
Source References: PMID:10199406, PMID:9636175
Notes: Single cell line from H3E transformant.
By antiphos H3 antibody staining, no stain so should be all HHT2 S10A. Also checked by Southern.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01889 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1; Nulli 4 (MPR1; mp-s, VII)
Proper citation: RRID:TSC_SD00832 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: twi11[∆?::neo3]/twi11[∆?::neo3] (mpr1-1, TWI11)
Affected Genes: TWI11 (TTHERM_00144830)
Genomic Alteration: Micronucleus: Part of TWI11 coding replaced with neo3 cassette, homozygous
Notes: Homozygous TWI11 KO heterokaryon partial coding sequence in MIC were replaced with neo3 cassette
.
mates withTWI11 KO1-4-R1
This strain was supposed to be mp-r, but thawed mp-s.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02857 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: CHX1[C3]/CHX1[C3]; MPR1[C3]/MPR1[C3]; pmr1-1/pmr1-1; MAT2/MAT2 (CHX1[C3];MPR1[C3]; pmr1-1; MAT2; cy-s, mp-s, pm-r, ?)
Source References: PMID:8725229, PMID:8601476
Notes: Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01252 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: wild type D (wild type D; wild type D, II)
Notes: See Figure 1 on page 13 of Methods in Cell Biology Volume 62, Tetrahymena thermophila 2000 (Editors D.J. Asai and J.D. Forney) for and excellent diagram explaining how the inbred strains are related to each other.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01496 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (twi1[HAn,3’neo3]; TWI1::HA-TWi1-neo3, pm-r, VII)
Affected Genes: TWI1 (TTHERM_01161040)
Genomic Alteration: Macronucleus: N-terminal HA tag on TWI1 with 3’ flanking neo3
Source References: PMID:12297043
Notes: CU428 was transformed with HA_TWI1-neo3 and endogenous TWI1 loci were completely replaced. See Mochizuki et al (2002) Cell 110,689-699. Chaker and Allis labs have some strains.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02069 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (?HHT3/hht3[5’neo2,FLAGn]; pm-r, VII)
Affected Genes: HTT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: N-terminal His- tag of HHT3, not sure if complete replacement
Source References: PMID:9343391, PMID:16908532
Notes: Somatic replace HHT3 gene with HHT3-Flag sequence. Neo2 in 5’ flanking of HHT3
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01374 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (HTAZ ; MTT1/mtt1[5’neo2,∆::HTAZ(K4R,K7R,K10R,K13R K16R,,K21R)]; pm-r, H2A.Z RRRRRRneo regulated by MTT1 promoter, VII)
Affected Genes: HTA3 (TTHERM_00143660)
Genomic Alteration: Macronucleus: inserted HTAZ with K4R, K7R, K10R, K13R K16R, , K21R Into MTT1 locus and neo2 in 5’flanking of MTT1
Source References: PMID:12665578, PMID:11430834
Notes: Cell has wt H2A.Z in its locus, and a mutated H2A.Z RRRRRR copy in MTT1 locus, neo2 cassette is inserted at 5’ of MTT, complete replacement ?. Somatic transformation.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02463 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (tcd2[∆?::neo4]/TCD2[5’bsr1]; Mix of pm-r and bs-r, II)
Affected Genes: HPL7 (TTHERM_00551070)
Genomic Alteration: Macronucleus: TCD2 was knocked out with neo4, then the wt TCD2 gene with a bsr1cassette upstream was somatically reintroduced into the cell
Notes: Using Complete somatic Neo4 knockout Tcd2 strains. Tcd2 gene was reintroduced with blasticidin cassette (H4-BSR-B) upstream.
H3K27 methylation was recovered.
Bsr/Bsr, Tcd2/Tcd2, Neo4/Neo4 This is what Wei wrote, not sure if the replacement was complete or if both resistance are still present.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02506 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: atu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1-1[Δ::neo1]/atu1[E441D, E443D, E445D,E446D,E447D ]; pm-r, mp-?, ?)
Affected Genes: ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: neo replaces ATU1 coding Macronucleus: ATU1 with E441D, E443D, E445D, E446D, E447D or neo replacing coding of some ATU1 genes
Source References: PMID:9037049, PMID:10831613
Notes: It is a progeny of conjugants of AAK02.7XAAKO5.5 transformed with pATU5D derived from pTUB100E3-PvuII (linearized)where the ATU1 gene is marked with Hae III and Pvu II sites and E441D, E443D, E445D,E446D,E447D. Slow growth.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02229 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: TTHERM_00137600[∆::NEO2] (TTHERM_00137600[∆::NEO2]; timing and location of basal body assembly, ?)
Affected Genes: Sas6b
Genomic Alteration: Micronucleus: TTHERM_00118700[∆::NEO2]
Source References: PMID:19158390
Notes: From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03323 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: MPR1-1/mpr1-1; TWI7/twi7[∆?::neo3] (mpr1-1; pm-r, ?)
Affected Genes: TWI7 (TTHERM_00600450)
Genomic Alteration: Micronucleus: Partial coding of TWI7 replaced by neo3
Notes: heterozygote TWI7 KO heterokaryon Partial TWI7 coding sequences were replaced by Neo3.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02870 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: oad1/oad1; chx1-1/chx1-1? (oad1; cy-s)
Source References: PMID:20189389, PMID:8401477
Notes: Submitted by Jacek Gaertig. Originally created by D. Pennock.
Proper citation: RRID:TSC_SD01784 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: homozygous HMG C KO with BSR (mp-r)
Affected Genes: HMGB (TTHERM_00660180)
Notes: Obtained thru 1st round exclusion of ∆B∆C gl KO. Genotype confirmed by cross with 427
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01388 Copy
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