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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1[∆::neo2]/ gtu1[∆::neo2]; mpr1-1/mpr1-1 (GTU1,mpr1-1; mp-r, VI)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: Neo2 replaces coding of GTU1
Source References: PMID:12356864
Notes: GTU germline heterokaryon, progeny from CU428 x B 2086 transformed with p∆GNII construct, can mate with CU428, same mating type as CU427
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01368 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht3[∆::neo2]/hht3[∆::neo2];hht4[∆::bsr1]/hht4[∆::bsr1]
(?)
Affected Genes: HHT3 (TTHERM_00016170), HHT4 (TTHERM_00016200)
Genomic Alteration: Micronucleus: HHT3 KO with neo2, HHT4 KO with bsr1
Source References: PMID:16908532
Notes: Germline double KO heterokaryon of HHT3(by neo2 cassette) and HHT4(by blasticidin cassette). Non* side. Mates with 3N4B-KO-*
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02214 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: possible heterozygote BSR KO (bsr-r, mp-r)
Affected Genes: MLH
Notes: Selected after germline shoot of mic LH-BSR construct. The genotype is to be confirmed after maturing and testing. Same as Mic LH BSR Gl KO -LH1, independent strain.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01406 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: dcl1[∆?::neo3]/dcl1[∆?::neo3] (dcl1[∆?::neo3]/dcl1[∆?::neo3]; MTT1/mtt1-?[5’ bsr1,∆,(::CNA1), GFPc]; bsr-r, pm-r, GFP tagged Cna1p, ?)
Affected Genes: CNA1 (TTHERM_00146340), DCL1 (TTHERM_00284230)
Genomic Alteration: Micronucleus: DCL1 replaced by neo3 Macronucleus: CNA1 GFP tagged replaces MTT1 coding in MTT1 locus, bsr1 cassete in MTT1 5’
Source References: PMID:16738316, PMID:15598983
Notes: ∆DCL1 homokaryon transformed with Bsr-MTT1-CNA1-GFP.
CNA1-GFP seq. was used to replace MTT1 coding seq. Bsr1 cassette was inserted at the 5’F seq. of MTT1 locus. The construct was transformed into mac.
Thaw cells without drugs, then grow them in 60-100ug/ml blasticidin, Use 0.5-1 ug/ml cadmium chloride to express and see the green centromere.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01806 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1 (chx1-1; cy-r, ory-r, MIX)
Genomic Alteration: Micronucleus: btu1-1::btu1-1M350K/btu1-1::btu1-1M350K (homozygous) Macronucleus: btu1-1::btu1-1M350K (homozygous)
Proper citation: RRID:TSC_SD00280 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (hht2[∆::neo2]; pm-r, VII)
Affected Genes: HHT2 (TTHERM_00189180)
Genomic Alteration: Macronucleus: Neo2 cassettes KO HHT2
Source References: PMID:9343391, PMID:16908532
Notes: HHT2 double knockout, neo 2 cassette
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01370 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; mpr1-1/mpr1-1 (mpr1-1; mp-r, II)
Affected Genes: HHT1 (TTHERM_00570560)
Genomic Alteration: Micronucleus: Neo KO of HHT1
Source References: PMID:15701804
Notes: The germline knockout heterokaryon of HHT1 gene with neo cassette completely replacing coding region. Three independent cell lines #1,#2,# 3.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02620 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: CHX1[C3]/CHX1[C3]; PMR1[C3]/PMR1[C3]; MAT3/MAT3 (CHX1[C3]; PMR1[C3]; MAT3; cy-s, pm-s, ?)
Source References: PMID:8725229, PMID:8601476
Notes: Resistance to 6-methylpurine was not scored for these panel members.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01253 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: N terminal HA tag in TWI 11 with neo3 homozygous (pm-r, ?)
Affected Genes: TWI11 (TTHERM_00144830)
Genomic Alteration: Macronucleus: N-terminal HA tag on Twi 11
Notes: Homozygous homokaryon HA -Twi 11. Mate with HA-Twi11 #1.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02067 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (snf2[∆::neo3/]snf2[∆::neo3], VII)
Affected Genes: SNF2 (TTHERM_00388250)
Genomic Alteration: Macronucleus: Neo 3 into coding of SNF2 in B2086
Notes: SNF2 family member involved in a variety of process including transcription regulation, DNA repair, DNA recombination and chromatin unwinding. The gene expression showed up- regulated during conjugation.
Pairs began to separate, the percent of anlagen is much lower than that of WT.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02189 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cen1-1[∆::NEO2] (cen1-1[∆::NEO2]::cen1-86; None., ?)
Affected Genes: CEN1 (TTHERM_00384910)
Genomic Alteration: Micronucleus: cen1-1[∆::NEO2] Macronucleus: cen1-1[∆::NEO2]::cen1-86
Source References: PMID:21562224
Notes: Alanine scanning allele rescue of cen1∆ - Cen1 D86A.
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03278 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (htb1[∆::neo2]/htb1[K115R]; htb2 [∆::neo2]; mp-r, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 into coding of HTB1 and HTB2 Macronucleus: HTB1 with K115R, HTB2 KO with neo2
Source References: PMID:19822522
Notes: New batch, rescue of progeny h∆∆HTB86B4A6 and D2A2. Not mating type II,VI,VII.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02469 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cna1[∆::neo2]/cna1[∆::neo2] (?)
Affected Genes: CNA1 (TTHERM_00146340)
Genomic Alteration: Micronucleus: Neo2 into CNA1
Source References: PMID:16738316
Notes: Germline CenPA KO heterokaryon. CenPA coding is replaced by neo2. Star side.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01933 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: in B2086 (MTT1/mtt1-?[5’ bsr1,∆,(::CNA1), GFPc]; Cna1p-GFPc, green centromeres, II)
Affected Genes: CNA1 (TTHERM_00146340), CenH3, CENP-A
Genomic Alteration: Macronucleus: GFP tagged CNA1 from the MTT1 locus
Source References: PMID:16738316
Notes: Centromeric H3 protein, green by IF. Partial mac replacement of MTT1 gene with GFP c-terminal tagged CNA1 gene. Thaw cells without drugs, then grow them in 60-100ug/ml blasticidin, Use 0.5-1 ug/ml cadmium chloride to express and see the green centromere.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01812 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (hho1[3’neo2, T35E, S43E, S45E, T47E, T54E, T60K,I64K, H65K,T67K,T69K]; pm-r, VII)
Affected Genes: HHO1 (TTHERM_00823720)
Genomic Alteration: Macronucleus: Change Threonine 35, 47, 54 and serine 43, 45 to glutamic acid (charge patch) and T60K, I64K, H65K, T67K, T69K
Source References: PMID:11972045, PMID:10983971
Notes: Complete somatic tranformant of CU428 cells with pHHO5K /neo construct. Phosphorylation sites 1- 5 of H1 changed to glutamic acid and 5 other sites changed to lysines, neo 2 cassette in 3’ flanking region for selection. See paper.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02476 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (?)
Notes: Collected by Paul Doerder on 8/21/2002 from the pond designated CRWP in Ridgway, PA (lat. 41 25.052, long. -78 51.876, elevation 1613 ft). Used Geko GPS.
Geographical area: Allegheny National Forest
Collection number: 453
No micronucleus present.
Proper citation: RRID:TSC_SD01547 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (htb1[∆::neo2]/htb1[A1G] ; htb2 [∆::neo2]; pm-r, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with A1G
Source References: PMID:19822522
Notes: ∆∆HTB knockout heterokaryons rescued by htb1-A1G.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02083 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hta1[∆::neo2]/hta1[∆::neo2]; hta2[∆::neo2]/ hta2[∆::neo2]; CHX1/CHX1;mpr1?/mpr1? (mp-r, II)
Affected Genes: HTA1 (TTHERM_00790790) + HTA2 (TTHERM_00316500)
Genomic Alteration: Micronucleus: Neo 2 knockout of HTAX and HTA1 genes
Source References: PMID:11430834, PMID:12665578
Notes: G115B5xG204F2. HTA1, HTA2 double germline knockout heterokaryon. Can mate with G4A1F14A, G4B3C1A
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01391 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2] (htb1[∆::neo2]; pm-r, ?)
Affected Genes: HTB1 (TTHERM_00633360)
Genomic Alteration: Micronucleus: neo2 KO of HTB1 Macronucleus: KO of HTB1 with neo2
Source References: PMID:19822522
Notes: ∆HTB1 knockout.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02082 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ift122[∆::neo3]/ift122[∆::neo3]; ift172[∆::neo3]/ift172[∆::neo3] (WT; pm-?, mp-?, ?)
Affected Genes: IFT122 (TTHERM_00694540), IFT172 (TTHERM_00089240)
Genomic Alteration: Micronucleus: Neo3 into coding of IFT122A and of IFT172
Source References: PMID:18211962, PMID:18199688
Notes: IFT122 and IFT172 double KO heterokaryon
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01790 Copy
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