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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00063163
Source Database: WormBase (WB)
Affected Genes: WBGene00001597(gld-3)
Genomic Alteration: WBGene00001597(gld-3)
Availability: unknown
Source References: EMPTY
Synonyms: gld-3(ust495[gld-3::GFP::3xFlag]) II.
Notes: GFP::3xFlag inserted into endogenous gld-3 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.|"Made_by: Xiaona Huang"
Proper citation: RRID:WB-STRAIN:WBStrain00063163 Copy
http://www.wormbase.org/db/get?name=WBStrain00063166
Source Database: WormBase (WB)
Affected Genes: WBGene00003993(pgl-2)
Genomic Alteration: WBGene00003993(pgl-2)
Availability: unknown
Source References: EMPTY
Synonyms: pgl-2(ust515[3xFlag::GFP::pgl-2]) III.
Notes: 3xFlag::GFP inserted into endogenous pgl-2 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.|"Made_by: Xiaona Huang"
Proper citation: RRID:WB-STRAIN:WBStrain00063166 Copy
http://www.wormbase.org/db/get?name=WBStrain00063167
Source Database: WormBase (WB)
Affected Genes: WBGene00021014(pid-4)
Genomic Alteration: WBGene00021014(pid-4)
Availability: unknown
Source References: EMPTY
Synonyms: pid-4(ust516[pid-4::GFP::3xFlag]) I.
Notes: GFP::3xFlag inserted into endogenous pid-4 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.|"Made_by: Xiaona Huang"
Proper citation: RRID:WB-STRAIN:WBStrain00063167 Copy
http://www.wormbase.org/db/get?name=WBStrain00063168
Source Database: WormBase (WB)
Affected Genes: WBGene00021555(pid-5)
Genomic Alteration: WBGene00021555(pid-5)
Availability: unknown
Source References: EMPTY
Synonyms: pid-5(ust517[pid-5::GFP::3xFlag]) V.
Notes: GFP::3xFlag inserted into endogenous pid-5 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.|"Made_by: Xiaona Huang"
Proper citation: RRID:WB-STRAIN:WBStrain00063168 Copy
http://www.wormbase.org/db/get?name=WBStrain00063208
Source Database: WormBase (WB)
Affected Genes: WBGene00001978(hmp-1)
Genomic Alteration: WBGene00001978(hmp-1)
Availability: unknown
Source References: EMPTY
Synonyms: hmp-1(jc58[hmp-1::mScarlet-1 + Lox511]) V.
Notes: Made_by: Joel M. Serre|"mScarlet tag inserted into endogenous hmp-1 locus by CRISPR/Cas9 genome editing. Reference: Serre JM, et al. PLoS Genet. 2023 Mar 3;19(3):e1010507. doi: 10.1371/journal.pgen.1010507. PMID: 36867663."
Proper citation: RRID:WB-STRAIN:WBStrain00063208 Copy
http://www.wormbase.org/db/get?name=WBStrain00063205
Source Database: WormBase (WB)
Availability: unknown
Source References: EMPTY
Synonyms: zjuEx2429.
Notes: Made_by: Jingxiu Xu|"zjuEx2429 [col-19p::BFP::8xMS2 + semo-1p::MCP::24Suntag + col-19p::scFv::sfGFP + myo-2p::GFP]. Pick GFP+ (pharynx) animals to maintain. MS2-based signal Amplification with Suntag System (MASS). Enhanced MS2-based single-molecule RNA imaging system utilizes Suntag signal amplifier. Suntag is a 19 amino acid protein tag that binds to its specific single-chain variable fragment (scFv) antibody. When all the required elements of the MASS (MS2, MCP, 24xSuntag, and scFv antibodies) are present, bright GFP foci will mark the tagged RNA. Reference: Hu Y, et al. eLife. 2023 Mar 3;12:e82178. doi: 10.7554/eLife.82178. PMID: 36867026."
Proper citation: RRID:WB-STRAIN:WBStrain00063205 Copy
http://www.wormbase.org/db/get?name=WBStrain00063151
Source Database: WormBase (WB)
Affected Genes: WBGene00004984(spn-4)
Genomic Alteration: WBGene00004984(spn-4)
Availability: unknown
Source References: EMPTY
Synonyms: spn-4(ust434[spn-4::GFP::3xFlag]) V.
Notes: GFP::3xFlag inserted into endogenous spn-4 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.|"Made_by: Xiaona Huang"
Proper citation: RRID:WB-STRAIN:WBStrain00063151 Copy
http://www.wormbase.org/db/get?name=WBStrain00063150
Source Database: WormBase (WB)
Affected Genes: WBGene00008061(mina-1)
Genomic Alteration: WBGene00008061(mina-1)
Availability: unknown
Source References: EMPTY
Synonyms: mina-1(ust433[mina-1::GFP::3xFlag]) I.
Notes: GFP::3xFlag inserted into endogenous mina-1 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.|"Made_by: Xiaona Huang"
Proper citation: RRID:WB-STRAIN:WBStrain00063150 Copy
http://www.wormbase.org/db/get?name=WBStrain00063155
Source Database: WormBase (WB)
Affected Genes: WBGene00001595(gld-1)
Genomic Alteration: WBGene00001595(gld-1)
Availability: unknown
Source References: EMPTY
Synonyms: gld-1(ust451[gld-1::GFP::3xFlag]) I.
Notes: GFP::3xFlag inserted into endogenous gld-1 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.|"Made_by: Xiaona Huang"
Proper citation: RRID:WB-STRAIN:WBStrain00063155 Copy
http://www.wormbase.org/db/get?name=WBStrain00063154
Source Database: WormBase (WB)
Affected Genes: WBGene00010281(mip-2)
Genomic Alteration: WBGene00010281(mip-2)
Availability: unknown
Source References: EMPTY
Synonyms: mip-2(ust450[mip-2::GFP::3xFlag]) V.
Notes: GFP::3xFlag inserted into endogenous mip-2 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.|"Made_by: Xiaona Huang"
Proper citation: RRID:WB-STRAIN:WBStrain00063154 Copy
http://www.wormbase.org/db/get?name=WBStrain00063159
Source Database: WormBase (WB)
Affected Genes: WBGene00001601(glh-4)
Genomic Alteration: WBGene00001601(glh-4)
Availability: unknown
Source References: EMPTY
Synonyms: glh-4(ust449[glh-4::GFP::3xFlag]) I.
Notes: GFP::3xFlag inserted into endogenous glh-4 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.|"Made_by: Xiaona Huang"
Proper citation: RRID:WB-STRAIN:WBStrain00063159 Copy
http://www.wormbase.org/db/get?name=WBStrain00063140
Source Database: WormBase (WB)
Affected Genes: WBGene00011580(ddx-19)
Genomic Alteration: WBGene00011580(ddx-19)
Availability: unknown
Source References: EMPTY
Synonyms: ddx-19(ust374[ddx-19::mCherry]) II.
Notes: mCherry inserted into endogenous ddx-19 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
Proper citation: RRID:WB-STRAIN:WBStrain00063140 Copy
http://www.wormbase.org/db/get?name=WBStrain00063148
Source Database: WormBase (WB)
Affected Genes: WBGene00015349(henn-1)
Genomic Alteration: WBGene00015349(henn-1)
Availability: unknown
Source References: EMPTY
Synonyms: henn-1(ust413[henn-1::GFP::3xFlag]) III.
Notes: GFP::3xFlag inserted into endogenous henn-1 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.|"Made_by: Xiaona Huang"
Proper citation: RRID:WB-STRAIN:WBStrain00063148 Copy
http://www.wormbase.org/db/get?name=WBStrain00063298
Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006745(unc-5)|WBGene00006789(unc-54)|WBGene00015551(adah-1)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006745(unc-5), WBGene00006789(unc-54), WBGene00015551(adah-1)
Availability: unknown
Source References: EMPTY
Synonyms: adah-1(hd7105 [loxP + myo-2p::GFP::unc-54 3 UTR + rps-27p::neoR::unc-54 3 UTR + loxP])/tmC25 [unc-5(tm9708)] IV.
Notes: Made_by: VH KO group|"Maintain by picking wild-type GFP+. Apparent homozygous lethal or sterile deletion balanced tmC25. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+ and Unc animals (tmC25 [unc-5(tm9708)] homozygotes). Derived from parental strains VH7105 and FX30257. hd7105 is a deletion of 4581 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation."
Proper citation: RRID:WB-STRAIN:WBStrain00063298 Copy
http://www.wormbase.org/db/get?name=WBStrain00063331
Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54)
Availability: unknown
Source References: EMPTY
Synonyms: F56B3.6(hd7168[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP]) IV.
Notes: Homozygous viable. Deletion of 2190 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Left flanking Sequence: TCAACTTCTGGACAACCGGCAGAAACGCCA; Right flanking sequence: GTAGGCACAAAGAAGGCGTAGGCCTCCTGG. sgRNA #1: TGGCGTTTCAGAGCTGCACG; sgRNA #2: AAAGCCAATTGTCTGAAAGG. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: VH KO group"
Proper citation: RRID:WB-STRAIN:WBStrain00063331 Copy
http://www.wormbase.org/db/get?name=WBStrain00063332
Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)|WBGene00018491(mdh-1)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54), WBGene00018491(mdh-1)
Availability: unknown
Source References: EMPTY
Synonyms: mdh-1(hd7169[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP]) V.
Notes: Homozygous viable. Deletion of 969 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Left flanking Sequence: CTAAGACCTTGAACAATCTTCCATTCGCCA; Right flanking sequence: CGGACATTCTGAAAATTTAGCAATTTACAC. sgRNA #1: TTCCCAGTTACCATCGAGGG; sgRNA #2: GACCAAAACGCGAAGTGGGG. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: VH KO group"
Proper citation: RRID:WB-STRAIN:WBStrain00063332 Copy
http://www.wormbase.org/db/get?name=WBStrain00063297
Source Database: WormBase (WB)
Affected Genes: WBGene00000254(bli-4)|WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00000254(bli-4), WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54)
Availability: unknown
Source References: EMPTY
Synonyms: F46F11.10(hd7096 [loxP + myo-2p::GFP::unc-54 3 UTR + rps-27p::neoR::unc-54 3 UTR + loxP])/hT2 [umnIs73] I; +/hT2 [bli-4(e937) let-?(h661)] III.
Notes: Made_by: VH KO group|"umnIs73 [myo-2p::mKate2 + NeoR, III: 9421936 (intergenic)] I. Pick viable fertile GFP+ and mKate2+ animals to maintain. Apparent homozygous lethal or sterile deletion balanced over mKate2 tagged hT2. Heterozygotes are wild-type GFP+ mKate2+, and segregate wild-type GFP+ mKate2+, GFP+ non-mKate2 (hd7096 homozygotes), lethal non-GFP mKate2+ hT2 homozygotes (arrest stage unknown) and dead eggs (aneuploids). Derived from parental strains VH7096 and CGC92. hd7096 is a 667 bp deletion with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation."
Proper citation: RRID:WB-STRAIN:WBStrain00063297 Copy
http://www.wormbase.org/db/get?name=WBStrain00063335
Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)|WBGene00020718(mrps-2)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54), WBGene00020718(mrps-2)
Availability: unknown
Source References: EMPTY
Synonyms: +/nT1 [umnls49] IV; mrps-2 (hd7170 [loxP + myo-2p::GFP::unc-54 3 UTR + rps-27p::neoR::unc-54 3 UTR + loxP])/nT1 V
Notes: Made_by: VH KO group|"umnIs49 [myo-2p::mKate2 + NeoR, V: 1005689 (intergenic)] IV. Pick viable fertile GFP+ and mKate2+ animals to maintain. Apparent homozygous lethal or sterile deletion balanced over nT1. Heterozygotes are wild-type GFP+ mKate2+, and segregate wild-type GFP+ mKate2+, Vul mKate2+ (nT1) and dead eggs. Derived from parental strains VH7170 and CGC63. hd7170 is a 966 bp deletion with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break. Left flanking Sequence: CAGAAAGAGCCTTCTCGACACGATTTTCCG; Right flanking sequence: TTCGAAAGTGGCAATCAGGAACTCTAACGA. sgRNA #1: AATGGTTACCTGCTGCGACG; sgRNA #2: GGTTGGGCAATACTCTCACG. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation."
Proper citation: RRID:WB-STRAIN:WBStrain00063335 Copy
http://www.wormbase.org/db/get?name=WBStrain00063336
Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)|WBGene00022152(atg-5)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54), WBGene00022152(atg-5)
Availability: unknown
Source References: EMPTY
Synonyms: atg-5(hd7174[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP]) I.
Notes: Homozygous viable. Deletion of 6488 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Left flanking Sequence: CTAAAGCCGAGATTACAAAGAATATGATGA; Right flanking sequence: GACCTTTTATAACTATTCACCCATACTAAT. sgRNA #1: AAGACGAGTCGGCACAGTTG; sgRNA #2: GTGAAGTTGTTATTGTACGG. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: VH KO group"
Proper citation: RRID:WB-STRAIN:WBStrain00063336 Copy
http://www.wormbase.org/db/get?name=WBStrain00063333
Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00004496(rps-27)|WBGene00006789(unc-54)
Genomic Alteration: WBGene00003514(myo-2), WBGene00004496(rps-27), WBGene00006789(unc-54)
Availability: unknown
Source References: EMPTY
Synonyms: cyp-33C8(hd7171[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP]) V.
Notes: Homozygous viable. Deletion of 2415 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Left flanking Sequence: AAACTTTCTCGGAGTTATCCCCTTCGATTT; Right flanking sequence: GGGAGAGGAGTAGGTCCCGGTGGTAAATTT. sgRNA #1: GTCGAGCGATGGAAGACCGG; sgRNA #2: GGAGAGTATTGCCGAACAGT. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.|"Made_by: VH KO group"
Proper citation: RRID:WB-STRAIN:WBStrain00063333 Copy
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