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pOSIP-KL-mcherry
 
Resource Report
Resource Website 		RRID:Addgene_116947 mcherry E. coli Kanamycin PMID:29765036 Vector Backbone:pOSIP-KL; Vector Types:; Bacterial Resistance:Kanamycin 2026-09-05 12:45:18 0
pLA230
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_11724 b-lactamase E. coli Kanamycin PMID:11602576 Positive control for mutagenesis experiments: reversion of the stop codon results in ampicillin resistance. The pGPS3 has a deletion from BglI to BglII that destroys the ampicillin resistance gene. The ampicillin resistance gene containing the stop codon was then cloned in between AflIII and KpnI. Please also see pGFPuv from BD Biosciences as another control for forward mutation assays. Catalog # 6079-1 http://www.clontech.com/US/Products/Fluorescent_Proteins_and_Reporters/Fluorescent_Proteins_by_Name/GFP_GFPuv_Fluorescent_Proteins?sitex=10020:22372:US . Backbone Marker:New England Biolabs; Backbone Size:2642; Vector Backbone:modified pGPS3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin GAA for Glu26 changed to TAA (premature stop codon introduced in beta-lactamase gene). 2026-09-05 12:45:20 1
pOmpW
 
Resource Report
Resource Website 		RRID:Addgene_112121 OmpW E. coli Ampicillin PMID:18641391 Backbone Size:5600; Vector Backbone:pET11A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin N-terminal signal sequence not included and replaced with Methionine. 2026-09-05 12:44:28 0
pOmpT
 
Resource Report
Resource Website 		RRID:Addgene_112123 OmpT E. coli Ampicillin PMID:18641391 Backbone Size:5636; Vector Backbone:pET11A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin N-terminal signal sequence not included and replaced with Methionine. 2026-09-05 12:44:28 0
N229A Lo-MetQ
 
Resource Report
Resource Website 		RRID:Addgene_118268 MetQ (N229A) E. coli Ampicillin PMID:30352853 Backbone Marker:Novagen; Backbone Size:5652; Vector Backbone:pET19b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Changed Asparagine (Asn) 229 to Alanine (Ala) 2026-09-05 12:45:30 0
N295A (EcN), F103A (EcI) MetNIQ in pBAD
 
Resource Report
Resource Website 		RRID:Addgene_118258 MetN (N295A) E. coli Ampicillin PMID:30352853 delta metNIQ E. coli strain BW2115 was a created in the Rees lab by modifying E. coli genomic DNA Backbone Marker:Thermo Scientific Invitrogen; Backbone Size:3963; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Changed Glutamic Acid (Glu) 295 to Alanine (Ala) 2026-09-05 12:45:30 0
N229A (EcQ) MetNIQ in pBAD
 
Resource Report
Resource Website 		RRID:Addgene_118257 MetN E. coli Ampicillin PMID:30352853 Backbone Marker:Thermo Scientific Invitrogen; Backbone Size:3963; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Changed Glutamic Acid (Glu) 295 to Alanine (Ala) 2026-09-05 12:45:30 0
N295A (EcN), M107A (EcI) MetNIQ in pBAD
 
Resource Report
Resource Website 		RRID:Addgene_118259 MetN (N295A) E. coli Ampicillin PMID:30352853 Backbone Marker:Thermo Scientific Invitrogen; Backbone Size:3963; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Changed Glutamic Acid (Glu) 295 to Alanine (Ala) 2026-09-05 12:45:30 0
N295A (EcN) MetNIQ in pBAD
 
Resource Report
Resource Website 		RRID:Addgene_118256 MetN (N295A) E. coli Ampicillin PMID:30352853 Backbone Marker:Thermo Scientific Invitrogen; Backbone Size:3963; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Changed Asparagine (Asn) 295 to Alanine (Ala) 2026-09-05 12:45:30 0
pET-2(KW)2-mCherry
 
Resource Report
Resource Website 		RRID:Addgene_112738 2(KW)2-mCherry E. coli Ampicillin PMID:26264666 Note: The mCherry insert contains a N9Q mutation. The depositor has confirmed this does not affect plasmid function. Backbone Marker:Novagen; Backbone Size:5706; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-05 12:44:35 0
pET-2HMG-eGFP
 
Resource Report
Resource Website 		RRID:Addgene_112737 2HMG-eGFP E. coli Ampicillin Backbone Marker:Novagen; Backbone Size:5706; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-09-05 12:44:35 0
pPB-R1R2-EM7neoPheS
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_112923 neoPheS E. coli Ampicillin PMID:30477585 Backbone Size:3470; Vector Backbone:pPB_LR5; Vector Types:Mammalian Expression, piggyBac transposon Gateway destination vector; Bacterial Resistance:Ampicillin 2026-09-05 12:44:37 1
pBLind
 
Resource Report
Resource Website 		RRID:Addgene_113109 RFP E. coli Kanamycin PMID:29596741 Vector Backbone:pBbE8K; Vector Types:Bacterial Expression, Cell-free; Bacterial Resistance:Kanamycin 2026-09-05 12:44:39 0
pAW218
 
Resource Report
Resource Website 		RRID:Addgene_113240 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-09-05 12:44:40 0
pAW204
 
Resource Report
Resource Website 		RRID:Addgene_113244 IHFa F13A E. coli Chloramphenicol PMID:28729350 Backbone Size:3600; Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol F13A 2026-09-05 12:44:40 0
pAW215
 
Resource Report
Resource Website 		RRID:Addgene_113237 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-09-05 12:44:40 0
pAW214
 
Resource Report
Resource Website 		RRID:Addgene_113236 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-09-05 12:44:40 0
pAW213
 
Resource Report
Resource Website 		RRID:Addgene_113235 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-09-05 12:44:40 0
pAW217
 
Resource Report
Resource Website 		RRID:Addgene_113239 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-09-05 12:44:40 0
pAW186
 
Resource Report
Resource Website 		RRID:Addgene_113184 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin R163A 2026-09-05 12:44:40 0

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