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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pBSV2H_Psyn-sgRNAflaB
 
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RRID:Addgene_149561 sgRNAflaB Synthetic Hygromycin PMID:33257311 Addgene QC identified an IS element present in the backbone sequence. The depositing lab does not believe this impacts the functional use of the plasmid but requesting scientists should validate expected behavior prior to experimentation. Backbone Marker:CJW lab; Vector Backbone:pBSV2H; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin 2026-09-05 12:49:29 0
pBSV2H_Psyn-sgRNAmreB
 
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RRID:Addgene_149567 sgRNAmreB Synthetic Hygromycin PMID:33257311 Backbone Marker:CJW lab; Vector Backbone:pBSV2H; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin 2026-09-05 12:49:29 0
pBSV2H_Psyn-sgRNArodA
 
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RRID:Addgene_149569 sgRNArodA Synthetic Hygromycin PMID:33257311 Backbone Marker:CJW lab; Vector Backbone:pBSV2H; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin 2026-09-05 12:49:29 0
pBbdCas9H(-10TC)_arr2
 
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RRID:Addgene_149587 Hygromycin PMID:33257311 Backbone Marker:CJW lab; Vector Backbone:pBbdCas9H(-10TC); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin 2026-09-05 12:49:29 0
pGWB2-CYCD3;1
 
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RRID:Addgene_140750 Cyclin D3;1 Arabidopsis thaliana Hygromycin PMID:31679162 Backbone Marker:15572; Vector Backbone:pGWB2; Vector Types:Plant Expression; Bacterial Resistance:Hygromycin 2026-09-05 12:48:56 0
pOSV805
 
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RRID:Addgene_126598 Hygromycin PMID:31175189 Backbone Size:5835; Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Hygromycin 2026-09-05 12:46:49 0
pJDC122
 
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RRID:Addgene_157876 tdTomato Hygromycin PMID:26934495 Vector Backbone:pJDC89; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-09-05 12:50:16 0
pRD393
 
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RRID:Addgene_168774 mEos3.2_H-NSdbd Synthetic Hygromycin PMID:34734265 Backbone Size:5625; Vector Backbone:pFPV27; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-09-05 12:51:46 0
pRD445
 
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RRID:Addgene_168775 mEos3.2 Synthetic Hygromycin PMID:34734265 Backbone Size:5625; Vector Backbone:pFPV27; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-09-05 12:51:46 0
pSBY2_ligD(FnCpf1)
 
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RRID:Addgene_104623 crRNA to ligD Synthetic Hygromycin PMID:30039929 Shuttle vector Mycobacterium smegmatis/E.coli Vector Backbone:pMN016; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin 2026-09-05 12:43:20 0
BB3cH_pGAP_23*_pLAT1_Cas9
 
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1+ mentions
RRID:Addgene_104907 HH - FS23 linker - HDV Other Hygromycin PMID:30737742 Additional Publication: Prielhofer, R. etal (2017) BMC Syst Biol. 11,123 (PMID:29221460) Vector Backbone:Episomal ARS/CEN Backbone; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Hygromycin 2026-09-05 12:43:22 1
BB3cH_pGAP_23*_pPFK300_Cas9
 
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1+ mentions
RRID:Addgene_104912 HH - FS23 linker - HDV Other Hygromycin PMID:30737742 Additional Publication: Prielhofer, R. etal (2017) BMC Syst Biol. 11,123 (PMID:29221460) Vector Backbone:Episomal ARS/CEN Backbone; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Hygromycin 2026-09-05 12:43:22 1
pML2424
 
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RRID:Addgene_117497 Hygromycin PMID:22885758 Addgene QC Next-generation sequencing identified sequence discrepancies relative to the Genbank file linked under Supplemental Documents. Plasmid is expected to function as described in the associated publication. Vector Backbone:pML2424; Vector Types:Deletion generation; Bacterial Resistance:Hygromycin 2026-09-05 12:45:23 0
pYUB1062-GFP
 
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RRID:Addgene_117695 Hygromycin PMID:30319771 This is a shuttle vector, designed for expression of the inserted gene in Mycobacterium smegmatis as well as allowing cloning and expressions using E. coli. This plasmid was constructed by Anajana Radhakrishnan from the pYUB1062 vector. pYUB1062 was donated by William R. Jacobs from the Department of Microbiology and Immunology at the Albert Einstein College of Medicine, New York, USA. References: this paper has been accepted in RSC Advances: A GFP-strategy for efficient recombinant protein overexpression and purification in Mycobacterium smegmatis: Anjana Radhakrishnan, Christopher M Furze, Mohd Syed Ahangar, Elizabeth Fullam Backbone Marker:pYUB1062 was donated by William R. Jacobs from the Department of Microbiology and Immunology at the Albert Einstein; Backbone Size:5800; Vector Backbone:pYUB1062; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-09-05 12:45:25 0
pMEG_BB3_YL18H_AC
 
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RRID:Addgene_117828 Hygromycin PMID:30698703 Contains 34 bp GoldenMOCS-Yali Linker AB Backbone Size:4649; Vector Backbone:pMEG_BB3_YL18H_AC; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Hygromycin 2026-09-05 12:45:26 0
pMST-1313
 
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RRID:Addgene_118076 pTEF1-cexA-tCYC1 A. niger Hygromycin PMID:30553933 Backbone Marker:Matthias Steiger; Vector Backbone:MST606; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin 2026-09-05 12:45:28 0
pDD132
 
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RRID:Addgene_119885 BbaJ23104_EYFP Hygromycin PMID:29366319 Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-09-05 12:45:45 0
pBSV2H
 
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RRID:Addgene_118229 Hygromycin PMID:33257311 Please visit https://www.biorxiv.org/content/early/2018/09/21/363390 for bioRxiv preprint. Addgene QC identified a partial duplication of the segment of the plasmid containing the HygR selectable marker. The plasmid is functional for transformation and selection in B. burgdorferi. This plasmid was functional for use as described in Takacs et al. 2018. Vector Backbone:pBSV2H; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-09-05 12:45:30 0
pVITRO-Fab_Quin-LUCID
 
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RRID:Addgene_119049 Fab_Quin-LUCID_part1 Synthetic Hygromycin PMID:28510347 Backbone Marker:Invivogen; Backbone Size:6295; Vector Backbone:pVITRO1-Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin 2026-09-05 12:45:38 0
pVITRO-Fab_MTX-LUCID
 
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RRID:Addgene_119047 Fab_MTX-LUCID_part1 Synthetic Hygromycin PMID:28510347 Backbone Marker:Invivogen; Backbone Size:6295; Vector Backbone:pVITRO1-Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin 2026-09-05 12:45:38 0

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