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Vector Backbone Description: Backbone Marker:VectorBuilder; Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:42056505
Comments: Please visit https://doi.org/10.1101/2025.07.23.666448 for bioRxiv preprint.
Proper citation: RRID:Addgene_253782 Copy
Vector Backbone Description: Backbone Marker:VectorBuilder; Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:42056505
Comments: Please visit https://doi.org/10.1101/2025.07.23.666448 for bioRxiv preprint.
Proper citation: RRID:Addgene_253768 Copy
Vector Backbone Description: Backbone Marker:Brett Stringer; Vector Backbone: lenti-sgRNA hygro (Addgene Plasmid #104991); Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:42365381
Comments: Adapted from lenti-sgRNA hygro (Plasmid #104991). Requires ccdB resistant competent cells
Proper citation: RRID:Addgene_235048 Copy
Vector Backbone Description: Backbone Marker:VectorBuilder; Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:42056505
Comments: Please visit https://doi.org/10.1101/2025.07.23.666448 for bioRxiv preprint.
Proper citation: RRID:Addgene_253780 Copy
Vector Backbone Description: Backbone Marker:VectorBuilder; Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:42056505
Comments: Please visit https://doi.org/10.1101/2025.07.23.666448 for bioRxiv preprint.
Proper citation: RRID:Addgene_253769 Copy
Vector Backbone Description: Backbone Marker:VectorBuilder; Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:42056505
Comments: Please visit https://doi.org/10.1101/2025.07.23.666448 for bioRxiv preprint.
Proper citation: RRID:Addgene_253778 Copy
Vector Backbone Description: Backbone Marker:VectorBuilder; Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:42056505
Comments: Please visit https://doi.org/10.1101/2025.07.23.666448 for bioRxiv preprint.
Proper citation: RRID:Addgene_253770 Copy
Vector Backbone Description: Backbone Marker:VectorBuilder; Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:42056505
Comments: Please visit https://doi.org/10.1101/2025.07.23.666448 for bioRxiv preprint.
Proper citation: RRID:Addgene_253773 Copy
Vector Backbone Description: Backbone Marker:VectorBuilder; Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:42056505
Comments: Please visit https://doi.org/10.1101/2025.07.23.666448 for bioRxiv preprint.
Proper citation: RRID:Addgene_253775 Copy
Vector Backbone Description: Backbone Marker:VectorBuilder; Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:42056505
Comments: Please visit https://doi.org/10.1101/2025.07.23.666448 for bioRxiv preprint.
Proper citation: RRID:Addgene_253777 Copy
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:42424437
Comments: pHAGE vector originally based on pHAGE-CAGS-W vector (Dr. Richard Mulligan, Harvard Institute of Medicine, Boston)
Proper citation: RRID:Addgene_259769 Copy
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:42424437
Comments: pHAGE vector originally based on pHAGE-CAGS-W vector (Dr. Richard Mulligan, Harvard Institute of Medicine, Boston)
Proper citation: RRID:Addgene_259774 Copy
Species: bacteria
Genetic Insert: aphA::cat
Vector Backbone Description: Backbone Marker:Skorupski, K., Taylor, R.K.; Backbone Size:4400; Vector Backbone:pKAS32; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:16750581
Comments: Contains cat which confers resistance to chloramphenicol.
Proper citation: RRID:Addgene_20870 Copy
Genetic Insert: Gateway Destination Vector
Vector Backbone Description: Backbone Size:6465; Vector Backbone:PB-CA; Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19252478
Proper citation: RRID:Addgene_20960 Copy
Genetic Insert: GateWay conversion cassette
Vector Backbone Description: Backbone Size:14820; Vector Backbone:pRosa26-PA; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19014667
Comments: pRosa26-DEST was made by inserting a GateWay conversion cassette into the XhoI site of pBigT, followed by transfer of PacI / AscI fragment of the resulting vector to the pRosa26-PA vector (see http://www.biomedcentral.com/1471-213X/1/4
for details on these vectors). The resulting vector resembles the construct that was used for the R26R Cre reporter mouse made by Phil Soriano in which the expression of lacZ is coupled to the endogenous Rosa26 locus via a splice acceptor site, but only after removal of the lox-STOP-lox cassette (which is actually the neo-R cassette used during the targeting). The only thing changed from the pBigT vectors and the R26R reporter is the cloning strategy, so the resulting knock-in should be as good as these systems, and as good (or as bad) as the use of the Rosa26 locus in any situation.
Stbl3 cells are advisable to use after users have done the LR recombination reaction.
See http://www.hgu.mrc.ac.uk/people/n.hastie_rosa.html
for more information and protocols for this plasmid.
Further advice from the depositing lab:
"Grow single colonies in Amp + Cam at 30 C, definitively not 37. Including the Cam should ensure that spontaneous recombinants that loose the Gateway cassette don't survive...
- Do minipreps and test with the double digest PacI / AscI. This should give 4.9 kb + 9.8 kb. If this is okay, the vector should be okay"
Please note that there are discrepancies between Addgene's quality control sequence and the theoretical sequence supplied by the depositing lab. These discrepancies should not affect the function of the construct.
Proper citation: RRID:Addgene_21189 Copy
Vector Backbone Description: Backbone Size:5808; Vector Backbone:pCS2+; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Comments: pCS2+ was digested with Stu I and ligated with the Reading Frame B Gateway cassette
Proper citation: RRID:Addgene_22423 Copy
Vector Backbone Description: Backbone Size:5800; Vector Backbone:pCS2+; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Comments: pCSDest was digested with PstI and XhoI to remove the attR2 site and was replaced with an attR3 site from pDESTR4-R3
Proper citation: RRID:Addgene_22424 Copy
Genetic Insert: BFP
Vector Backbone Description: Backbone Size:3125; Vector Backbone:pBS; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27729526
Proper citation: RRID:Addgene_86457 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Marker:PMID: 17878951; Backbone Size:8537; Vector Backbone:pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17878951
Comments: Gateway destination vector for electroporation experiments.
Proper citation: RRID:Addgene_186414 Copy
Vector Backbone Description: Backbone Size:10576; Vector Backbone:FUCGW; Vector Types:Lentiviral, Gateway: DEST vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: FUCGW (PMID 16682621) was a gift from Dr. John K. Lee. FUCGW-DEST construct was cloned by Dr. Michael D. Nyquist.
Proper citation: RRID:Addgene_208408 Copy
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