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Vector Backbone Description: Vector Backbone:pML2424; Vector Types:Deletion generation; Bacterial Resistance:Hygromycin
Defining Citation: PMID:22885758
Comments: Addgene QC Next-generation sequencing identified sequence discrepancies relative to the Genbank file linked under Supplemental Documents. Plasmid is expected to function as described in the associated publication.
Proper citation: RRID:Addgene_117497 Copy
Vector Backbone Description: Backbone Marker:pYUB1062 was donated by William R. Jacobs from the Department of Microbiology and Immunology at the Albert Einstein; Backbone Size:5800; Vector Backbone:pYUB1062; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30319771
Comments: This is a shuttle vector, designed for expression of the inserted gene in Mycobacterium smegmatis as well as allowing cloning and expressions using E. coli.
This plasmid was constructed by Anajana Radhakrishnan from the pYUB1062 vector. pYUB1062 was donated by William R. Jacobs from the Department of Microbiology and Immunology at the Albert Einstein College of Medicine, New York, USA.
References: this paper has been accepted in RSC Advances:
A GFP-strategy for efficient recombinant protein overexpression and purification in Mycobacterium smegmatis: Anjana Radhakrishnan, Christopher M Furze, Mohd Syed Ahangar, Elizabeth Fullam
Proper citation: RRID:Addgene_117695 Copy
Vector Backbone Description: Backbone Size:4649; Vector Backbone:pMEG_BB3_YL18H_AC; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30698703
Comments: Contains 34 bp GoldenMOCS-Yali Linker AB
Proper citation: RRID:Addgene_117828 Copy
Vector Backbone Description: Backbone Size:5835; Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31175189
Proper citation: RRID:Addgene_126598 Copy
Species: A. niger
Genetic Insert: pTEF1-cexA-tCYC1
Vector Backbone Description: Backbone Marker:Matthias Steiger; Vector Backbone:MST606; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30553933
Proper citation: RRID:Addgene_118076 Copy
Vector Backbone Description: Vector Backbone:pBSV2H; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33257311
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/21/363390 for bioRxiv preprint.
Addgene QC identified a partial duplication of the segment of the plasmid containing the HygR selectable marker. The plasmid is functional for transformation and selection in B. burgdorferi. This plasmid was functional for use as described in Takacs et al. 2018.
Proper citation: RRID:Addgene_118229 Copy
Species: Synthetic
Genetic Insert: Fab_Quin-LUCID_part1
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:6295; Vector Backbone:pVITRO1-Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:28510347
Proper citation: RRID:Addgene_119049 Copy
Species: Synthetic
Genetic Insert: Fab_MTX-LUCID_part1
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:6295; Vector Backbone:pVITRO1-Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:28510347
Proper citation: RRID:Addgene_119047 Copy
Genetic Insert: BbaJ23104_EYFP
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:29366319
Proper citation: RRID:Addgene_119885 Copy
Genetic Insert: tdTomato
Vector Backbone Description: Vector Backbone:pJDC89; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:26934495
Proper citation: RRID:Addgene_157876 Copy
Species: Synthetic
Genetic Insert: sgRNAflaB
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2H; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33257311
Comments: Addgene QC identified an IS element present in the backbone sequence. The depositing lab does not believe this impacts the functional use of the plasmid but requesting scientists should validate expected behavior prior to experimentation.
Proper citation: RRID:Addgene_149561 Copy
Species: Synthetic
Genetic Insert: sgRNAmreB
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2H; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149567 Copy
Species: Synthetic
Genetic Insert: sgRNArodA
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBSV2H; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149569 Copy
Vector Backbone Description: Backbone Marker:CJW lab; Vector Backbone:pBbdCas9H(-10TC); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33257311
Proper citation: RRID:Addgene_149587 Copy
Species: Arabidopsis thaliana
Genetic Insert: Cyclin D3;1
Vector Backbone Description: Backbone Marker:15572; Vector Backbone:pGWB2; Vector Types:Plant Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31679162
Proper citation: RRID:Addgene_140750 Copy
Species: Synthetic
Genetic Insert: crRNA to ligD
Vector Backbone Description: Vector Backbone:pMN016; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30039929
Comments: Shuttle vector Mycobacterium smegmatis/E.coli
Proper citation: RRID:Addgene_104623 Copy
Species: Other
Genetic Insert: HH - FS23 linker - HDV
Vector Backbone Description: Vector Backbone:Episomal ARS/CEN Backbone; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30737742
Comments: Additional Publication: Prielhofer, R. etal (2017) BMC Syst Biol. 11,123 (PMID:29221460)
Proper citation: RRID:Addgene_104907 Copy
Species: Other
Genetic Insert: HH - FS23 linker - HDV
Vector Backbone Description: Vector Backbone:Episomal ARS/CEN Backbone; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30737742
Comments: Additional Publication: Prielhofer, R. etal (2017) BMC Syst Biol. 11,123 (PMID:29221460)
Proper citation: RRID:Addgene_104912 Copy
Genetic Insert: GFP (including ATG start codon)
Vector Backbone Description: Backbone Marker:Koen De Smet Imperial College Medical School; Backbone Size:5497; Vector Backbone:pSODIT; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:11250077
Comments: Plasmid can also be grown in mycobacteria.
Proper citation: RRID:Addgene_31851 Copy
Vector Backbone Description: Backbone Marker:Koen De Smet Imperial College Medical School; Backbone Size:8221; Vector Backbone:pACE; Vector Types:Bacterial Expression, Escherichia coli-Mycobacterium shuttle plasmid; Bacterial Resistance:Hygromycin
Defining Citation: PMID:12754249
Comments: Plasmid can also be grown in mycobacteria.
Also see PMID 15342574 for additional reference.
Please note that Addgene were unable to verify the His tag in this construct. In addition there is a 1bp mismatch in our ACEpro-F sequence with the available NCBI reference. Depositing lab believes the construct is functional.
Proper citation: RRID:Addgene_31853 Copy
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