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Species: bacteria
Genetic Insert: RecA
Vector Backbone Description: Backbone Size:9700; Vector Backbone:Ts vector; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:20885380
Proper citation: RRID:Addgene_249731 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Marker:Nova Lifetech Inc.; Backbone Size:10682; Vector Backbone:pRK415; Vector Types:Bacterial Expression, Synthetic Biology, very low copy; Bacterial Resistance:Tetracycline
Comments: Please visit https://doi.org/10.64898/2026.02.27.708671 for bioRxiv preprint.
Proper citation: RRID:Addgene_248204 Copy
Species: Synthetic
Genetic Insert: PylRS-C6a
Vector Backbone Description: Backbone Marker:in-house design and optimization; Vector Backbone:pGS1T; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41974833
Comments: Please visit https://doi.org/10.1101/2025.02.09.636911 for bioRxiv preprint.
Proper citation: RRID:Addgene_251555 Copy
Genetic Insert: SceI
Vector Backbone Description: Vector Backbone:pSW-Tc; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:36216579
Proper citation: RRID:Addgene_252220 Copy
Species: Streptococcus pasteurianus
Genetic Insert: dcas9
Vector Backbone Description: Vector Backbone:miniCTX1; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41670347
Proper citation: RRID:Addgene_253726 Copy
Species: Synthetic
Genetic Insert: MbPylRS-4ThzA
Vector Backbone Description: Backbone Marker:in-house design and optimization; Vector Backbone:pOS1T; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41885601
Comments: Please visit https://doi.org/10.1101/2025.11.04.686677 for bioRxiv preprint.
Proper citation: RRID:Addgene_253668 Copy
Species: Synthetic
Genetic Insert: chPylRS-4OxzA
Vector Backbone Description: Backbone Marker:in-house design and optimization; Vector Backbone:pOS1T; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41885601
Comments: Please visit https://doi.org/10.1101/2025.11.04.686677 for bioRxiv preprint.
Proper citation: RRID:Addgene_253663 Copy
Genetic Insert: Mb(IPYE)PylRS-τMH
Vector Backbone Description: Backbone Marker:in-house design and optimization; Vector Backbone:pOS1T; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41885601
Comments: Please visit https://doi.org/10.1101/2025.11.04.686677 for bioRxiv preprint.
Proper citation: RRID:Addgene_253665 Copy
Species: Synthetic
Genetic Insert: MmPylRS(8_2)
Vector Backbone Description: Backbone Marker:in-house design and optimization; Vector Backbone:pOS1T; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41885601
Comments: Please visit https://doi.org/10.1101/2025.11.04.686677 for bioRxiv preprint.
Proper citation: RRID:Addgene_253670 Copy
Species: Synthetic
Genetic Insert: chPylRS-2E5MH
Vector Backbone Description: Backbone Marker:in-house design and optimization; Vector Backbone:pOS1T; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41885601
Comments: Please visit https://doi.org/10.1101/2025.11.04.686677 for bioRxiv preprint.
Proper citation: RRID:Addgene_253657 Copy
Species: Synthetic
Genetic Insert: AIcrVIA3
Vector Backbone Description: Vector Backbone:pPF4213; Vector Types:Transposon insertion mutagenesis vector; Bacterial Resistance:Tetracycline
Comments: Please visit https://doi.org/10.64898/2025.12.19.695335 for bioRxiv preprint.
Proper citation: RRID:Addgene_254226 Copy
Species: Phage PCH45
Genetic Insert: uvsX & linker
Vector Backbone Description: Vector Backbone:pPF4213; Vector Types:Transposon insertion mutagenesis vector; Bacterial Resistance:Tetracycline
Comments: Please visit https://doi.org/10.64898/2025.12.19.695335 for bioRxiv preprint.
Proper citation: RRID:Addgene_254225 Copy
Vector Backbone Description: Vector Backbone:pSynORI; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41629321
Comments: J23119-Repressor (F4m1)-RNA Primer (-68nt)-Target (F4m1)-J23110-parDE-parCBA-TetR
Please note that the depositor's annotated reference file has minor differences compared to the Addgene verified sequence. The depositing lab confirms that the single nucleotide mismatch within the ‘RNAII’ annotated feature (nt #2527) is not expected to alter the plasmid's function.
Please refer to the the Addgene sequence for the most accurate result.
Proper citation: RRID:Addgene_253208 Copy
Vector Backbone Description: Backbone Marker:Kevin Roberts and George Salmond, Cambridge University; Backbone Size:5664; Vector Backbone:pDS1028; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:34349283
Proper citation: RRID:Addgene_254603 Copy
Genetic Insert: KJ1C bacterial Strain
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:19798082
Comments: F- ∆hisB463 ∆(gpt-proAB-arg-lac)XIII zaj::Tn10
Proper citation: RRID:Addgene_21874 Copy
Vector Backbone Description: Backbone Size:4241; Vector Backbone:pBMTL-4; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:16496398
Proper citation: RRID:Addgene_22814 Copy
Species: Synthetic
Genetic Insert: TLS2
Vector Backbone Description: Backbone Marker:Cermak et al. 2011; Vector Backbone:pHD1; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41123679
Comments: The Addgene NGS sequence differs from the reference sequence attached: it is missing a section of backbone, but the depositor has confirmed this does not affect plasmid function.
Proper citation: RRID:Addgene_245882 Copy
Species: Synthetic
Genetic Insert: PtALSgRNA
Vector Backbone Description: Backbone Marker:Cermak et al. 2011; Vector Backbone:pHD1; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41123679
Comments: The Addgene NGS sequence differs from the reference sequence attached: it is missing a section of backbone, but the depositor has confirmed this does not affect plasmid function.
Proper citation: RRID:Addgene_245881 Copy
Species: Synthetic
Genetic Insert: CsALS_gRNA1.1
Vector Backbone Description: Backbone Marker:Cermak et al. 2011; Vector Backbone:pHD1; Vector Types:Plant Expression, CRISPR, Golden Gate entry vector to expressing the 1st gRNA; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41123679
Proper citation: RRID:Addgene_245875 Copy
Species: Synthetic
Genetic Insert: TLS2
Vector Backbone Description: Backbone Marker:Cermak et al. 2011; Vector Backbone:pHD1; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:41123679
Comments: The Addgene NGS sequence differs from the reference sequence attached: it is missing a section of backbone, but the depositor has confirmed this does not affect plasmid function.
Proper citation: RRID:Addgene_245879 Copy
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