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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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KG#81 Resource Report Resource Website |
RRID:Addgene_110876 | myo-3 promoter | Caenorhabditis elegans | Ampicillin | PMID:15489510 | Always streak out this culture for single colonies and do minipreps on >= 4 clones (~1/2 of colonies will be right). Choose small colonies after 18 - 20h growth. DNA yeild is low (~170 ng/ ul x 30 ul for a Qiagen 3 ml miniprep). Used Pfu Ultra polymerase and primers engineered with restriction sites to amplify the 3.8 Kb acy-1(P260S) cDNA coding region from KG#63 and cloned into Age I/ Xho I cut pPD96.52 (myo-3:: expression vector). Transformed into XL1-Blue electrocompetent cells. Miniprepped 8 clones to find those with correct size insert and vector, then submitted 2 clones for sequence. Chose one clone that has correct sequence and make glycerol stock and save the DNA. Features of the expression construct: This expression construct has a promoter (myo-3) that will drive strong expression of acy-1 (P260S) cDNA in the body wall muscle cells. The myo-3 promoter is generally active in body muscles (body wall, vulval, and intestine associated muscles including the anal depressor [Okkema et al., 1993 Genetics; Ardizzi and Epstein 1987 JCB]. Other features of the expression vector include the unc-54 3' end including the poly A addition signal, and 3 introns (1 in 5' UTR, 1 just upstream of the unc-54 3' end, and 1 inserted in the unc-54 3' end). These introns help provide more uniform and robust expression, especially if you are expressing a cDNA with no introns (as we are) as opposed to a gene with introns. The sequence AAAA is engineered just after the 5' restriction site and just before the initiator ATG to provide a consensus translation start site. The sequence AT follows the stop codon and immediately precedes the 3' restriction site to provide a consensus post - stop codon sequence. Note: the acy-1 cDNA from which this clone was produced corresponds to the single acy-1 cDNA predicted by wormbase, which is F17C8.1 except for the gf mutation in this clone. | Backbone Size:2617; Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:15:06 | 0 | |
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p141 pETF-GST RanBP10 G308R Resource Report Resource Website |
RRID:Addgene_11094 | RanBP10 | Mus musculus | Kanamycin | Backbone Size:6000; Vector Backbone:pETF-GST; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | G308R | 2026-09-12 02:15:06 | 0 | ||
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KG#883 Resource Report Resource Website |
RRID:Addgene_110913 | 5X Myc-Spacers-Auxin Inducible Degron for N-terminal attachment | Arabidopsis thaliana | Ampicillin | Submitted the 5X Myc-Spacers+Degron sequence (see below) to Genscript for GenePlus Economy synthesis and cloning into the EcoRV site of pUC57. | Backbone Size:2710; Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:15:06 | 0 | ||
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KG#83 Resource Report Resource Website |
RRID:Addgene_110877 | rab-3 promoter | Caenorhabditis elegans | Ampicillin | PMID:15489510 | Always streak out this culture for single colonies and do minipreps on >= 4 clones (~1/2 of colonies will be right). Choose small colonies after 18 - 20h growth. DNA yeild is low (~170 ng/ ul x 30 ul for a Qiagen 3 ml miniprep). Used Age I/ Xho I to cut out the 3800 bp acy-1(P260S) cDNA from KG#81 and cloned into the like-digested rab-3 expression vector KG#59 (4900 bp). Transformed into XL1-Blue electrocompetent cells. Miniprepped 4 clones to find one with correct size insert and vector and made glycerol stock. Features of the construct: This expression construct has a promoter (rab-3) that will drive strong expression of the acy-1(P260S) cDNA in the nervous system. An unc-54 3' end containing a poly A addition signal is downstream of the MCS to provide a 3' UTR for the transcript. Three introns in the UTR's are included to help expression (1 in the 5' UTR, 1 just upstream of the unc-54 3' end, and 1 in the unc-54 3' end sequence). This construct includes only the acy-1 coding region. The sequence AAAA is engineered just after the 5' restriction site and just before the initiator ATG. An AT immediately follows the TAA stop codon. Note: the acy-1 cDNA from which this clone was produced corresponds to the single acy-1 cDNA predicted by wormbase, which is F17C8.1 except for the gf mutation in this clone. | Backbone Size:2617; Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:15:06 | 0 | |
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KG#84 Resource Report Resource Website |
RRID:Addgene_110878 | unc-17beta promoter | Caenorhabditis elegans | Ampicillin | PMID:15489510 | Used Age I/ Xho I to cut out the 3800 bp acy-1(P260S) cDNA from KG#81 and cloned into the like-digested unc-17b expression vector KG#65 (4200 bp). Transformed into XL1-Blue electrocompetent cells. Miniprepped 4 clones to find one with correct size insert and vector and made glycerol stock. The insert was previously checked by sequencing and contains only the P260S mutation. Features of the construct: This expression construct has a promoter (unc-17beta) that will drive strong expression of the acy-1(P260S) cDNA in the A and B classes of ventral cord motor neurons + AS's, but not VC's or cholinergic neurons in the head or tail. The vector contains a "decoy" (see 1995 vector kit documentation) upstream of the promoter to reduce background expression in the gut and pharynx from read-through transcription. An unc-54 3' end containing a poly A addition signal is downstream of the MCS to stop transcription and provide a 3' UTR for the transcript. Three introns in the UTR's are included to give stable and uniform expression (1 in the 5' UTR, 1 just upstream of the unc-54 3' end, and 1 in the unc-54 3' end sequence). This construct includes only the coding region. The sequence AAAA is engineered just after the 5' restriction site and just before the initiator ATG. An AT immediately follows the TAA stop codon for a consensus stop site. Note: the acy-1 cDNA from which this clone was produced corresponds to the single acy-1 cDNA predicted by wormbase, which is F17C8.1. | Backbone Size:2617; Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:15:06 | 0 | |
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KG#707 Resource Report Resource Website |
RRID:Addgene_110911 | sup-1 minigene | Caenorhabditis elegans | Ampicillin | PMID:30254025 | Made by IDT Custom Gene Synthesis. This sup-1 minigene can be inserted in C. elegans introns as a gene editing marker. The minigene begins with the 491 bp unc-17beta promoter. This is an engineered derivative of the full unc-17 promoter that drives expression specifically in cholinergic motor neurons of the ventral nerve cord (AS, DA, DB, VA, VB neuron classes) and not in cholinergic neurons elsewhere in the animal (Jim Rand, personal communication). The sup-1 portion of the minigene contains a single, small native intron (we removed a second, much larger intron) and includes the e995 suppressor mutation that rescues the unc-17(e245) mutant phenotype. The minigene finishes with the C. briggsae unc-119 3’ UTR, previously used in the unc-119 marker gene (FROKJAER-JENSEN et al. 2008). After searching the reverse orientation of the minigene sequence with the consensus spice acceptor sequence WTNYAG, we replaced 11 potential splice acceptors in the coding region, intron, and 3’ UTR with silent mutations. The AT content of the minigene is 63%, which approaches the 70% average for introns (BLUMENTHAL AND STEWARD 1997). The total size of the sup-1 minigene is 1112 bp. | Backbone Size:2124; Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:15:06 | 0 | |
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KG#908 Resource Report Resource Website |
RRID:Addgene_110914 | rab-3 promoter | Caenorhabditis elegans | Ampicillin | We used Q5 polymerase and primers engineered with restriction sites to amplify the 1885 bp TIR1 sequence from pLZ31 and cloned it into Nhe I/ Kpn I cut KG#59 (4.9 Kb). Transformed into XL1-Blue electrocompetent cells. Did Qiagen preps on 2 clones to find one with correct size insert for sequence verification. Features of the construct: The rab-3:: promoter drives expression pan-neuronally. AAAA provides a consensus ribosome binding site. TAA AT provides a consensus stop translation site. This is the Arabidopsis thaliana (mustard weed) TIR1 sequence published in the Zhang et al., 2015 Auxin Inducible Degradation paper from the Dernburg lab. It has been codon optimized for C. elegans and contains 2 synthetic introns to boost expression. It also contains 2 point mutations (D170E and M473L) shown to increase the affinity of the Arabidopsis TIR1 protein for its substrates and to increase auxin sensitivity without causing auxin-independent activity. There are also introns in the 5' and 3' UTR in this vector. | Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:15:06 | 0 | ||
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BSP179 Resource Report Resource Website |
RRID:Addgene_110915 | Kanamycin | PMID:33693300 | Backbone Marker:Mendenhall Lab; Vector Backbone:BSP179; Vector Types:Worm Expression, Synthetic Biology, Yeast Homologous Recombination cloning for Universal MosSCI; Bacterial Resistance:Kanamycin | 2026-09-12 02:15:06 | 0 | ||||
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plko-tet-on puromycin-shMTH1 Resource Report Resource Website |
RRID:Addgene_110918 | MTH1 | Homo sapiens | Ampicillin | PMID:25023700 | The Rai lab uses XL-10 gold ultracompetent cells as the growth strain, but DH5alpha should work fine too | Backbone Marker:PUBMED ID: 19177017; Vector Backbone:pLKO-Tet-on (puromycin); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-12 02:15:06 | 0 | |
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PF3D7_0526900-COMP-blac-flag-his Resource Report Resource Website |
RRID:Addgene_110985 | transmembrane emp24 domain-containing protein, putative | Synthetic | Ampicillin | PMID:33515807 | Backbone Marker:pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute; Backbone Size:7661; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | All NXT/S sites mutated to NXA, where X is any amino acid except Proline | 2026-09-12 02:15:07 | 0 | |
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PF3D7_0422100-COMP-blac-flag-his Resource Report Resource Website |
RRID:Addgene_110984 | transmembrane emp24 domain-containing protein, putative | Synthetic | Ampicillin | PMID:33515807 | Backbone Marker:pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute; Backbone Size:7661; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | All NXT/S sites mutated to NXA, where X is any amino acid except Proline | 2026-09-12 02:15:07 | 0 | |
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Rlf 328E-CAAX in pBabepuro Resource Report Resource Website |
RRID:Addgene_11111 | Rlf | Mus musculus | Ampicillin | PMID:15950903 | Backbone Size:5100; Vector Backbone:pbabepuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 328E CAAX deletion | 2026-09-12 02:15:08 | 0 | |
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PF3D7_0624400-COMP-blac-flag-his Resource Report Resource Website |
RRID:Addgene_111022 | conserved Plasmodium protein | Synthetic | Ampicillin | PMID:33515807 | Backbone Marker:pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute; Backbone Size:7661; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | All NXT/S sites mutated to NXA, where X is any amino acid except Proline | 2026-09-12 02:15:07 | 0 | |
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pALOD4 Resource Report Resource Website 1+ mentions |
RRID:Addgene_111026 | Domain 4 of Anthrolysin O | Bacillus Anthracis | Ampicillin | PMID:25809258 | Backbone Marker:Invitrogen; Backbone Size:2887; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Mutations: S404C, C472A | 2026-09-12 02:15:07 | 7 | |
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POFUT2-COMP-blac-flag-his Resource Report Resource Website |
RRID:Addgene_111024 | GDP fucose protein O-fucosyltransferase 2 (POFUT2) | Synthetic | Ampicillin | PMID:33515807 | Backbone Marker:pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute; Backbone Size:7661; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | All NXT/S sites mutated to NXA, where X is any amino acid except Proline | 2026-09-12 02:15:07 | 0 | |
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pALOD4 non-binding mutant Resource Report Resource Website |
RRID:Addgene_111027 | Domain 4 of Anthrolysin O | Bacillus Anthracis | Ampicillin | PMID:25809258 | Backbone Marker:Invitrogen; Backbone Size:2887; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Mutations: S404C, C472A, G501A, T502A, T503A, L504A, Y505A, P506A | 2026-09-12 02:15:07 | 0 | |
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mTalin2_F0 Resource Report Resource Website |
RRID:Addgene_111028 | TLN2 | Mus musculus | Ampicillin | PMID:29723415 | Backbone Size:5362; Vector Backbone:pET151TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:15:07 | 0 | ||
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pCR1265 Resource Report Resource Website 1+ mentions |
RRID:Addgene_111094 | Ampicillin | PMID:30054595 | Consensus CDS were PCR amplified from gBlocks or from cDNA clones and Gibson cloned into pLentiX1 | Vector Backbone:pLentiXI; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-12 02:15:08 | 1 | |||
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pCR1075 Resource Report Resource Website |
RRID:Addgene_111093 | eGFP | Homo sapiens | Ampicillin | PMID:30054595 | Consensus CDS were PCR amplified from gBlocks or from cDNA clones and Gibson cloned into pLentiX1 | Vector Backbone:pLentiXI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:15:08 | 0 | |
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PF3D7_1404900-COMP-blac-flag-his Resource Report Resource Website |
RRID:Addgene_111018 | conserved Plasmodium protein | Synthetic | Ampicillin | PMID:33515807 | Backbone Marker:pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute; Backbone Size:7661; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | All NXT/S sites mutated to NXA, where X is any amino acid except Proline | 2026-09-12 02:15:07 | 0 |
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