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Vector Backbone Description: Vector Backbone:pDEST17; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:38810650
Comments: Please visit https://doi.org/10.1101/2022.02.26.482115 for bioRxiv preprint. Also see Chen and Harel et al. 2024 (https://doi.org/10.1016/j.devcel.2024.04.014).
Proper citation: RRID:Addgene_166145 Copy
Species: Other
Genetic Insert: P2A_v1.1; VexGFP_v1.1; T2A_v1.1; BlastR_v1.1; Stop
Vector Backbone Description: Vector Backbone:pUC57-Kan; Vector Types:Fragment; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38484704
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216070 Copy
Species: Synthetic
Genetic Insert: mRuby3
Vector Backbone Description: Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38810650
Comments: Please visit https://doi.org/10.1101/2022.02.26.482115 for bioRxiv preprint. Also see Chen and Harel et al. 2024 (https://doi.org/10.1016/j.devcel.2024.04.014).
Proper citation: RRID:Addgene_166144 Copy
Species: Streptococcus pyogenes
Genetic Insert: WPRE_v1; U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v4 [Sp]; 3'LTR; bGHpolyA_v1
Vector Backbone Description: Vector Backbone:pUC57-Kan; Vector Types:Fragment; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38484704
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216073 Copy
Species: Staphylococcus aureus
Genetic Insert: U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v1 [Sa]; terminator_v1
Vector Backbone Description: Vector Backbone:pUC57-Kan; Vector Types:CRISPR, Fragment; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38484704
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216072 Copy
Vector Backbone Description: Vector Backbone:pRDA_512; Vector Types:Mammalian Expression, Lentiviral, Destination; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38484704
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216075 Copy
Species: Streptococcus pyogenes
Genetic Insert: WPRE_v1; U6_v1; BsmBI_v0; BsmBI_v5; trRNA_v6 [Sp]; 3'LTR; bGHpolyA_v1
Vector Backbone Description: Vector Backbone:pUC57-Kan; Vector Types:Fragment; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38484704
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216074 Copy
Vector Backbone Description: Vector Backbone:pDEST17; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:38810650
Comments: Please visit https://doi.org/10.1101/2022.02.26.482115 for bioRxiv preprint. Also see Chen and Harel et al. 2024 (https://doi.org/10.1016/j.devcel.2024.04.014).
Proper citation: RRID:Addgene_166148 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38810650
Comments: Please visit https://doi.org/10.1101/2022.02.26.482115 for bioRxiv preprint. Also see Chen and Harel et al. 2024 (https://doi.org/10.1016/j.devcel.2024.04.014).
Proper citation: RRID:Addgene_166143 Copy
Vector Backbone Description: Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:38810650
Comments: This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction.
Please visit https://doi.org/10.1101/2022.02.26.482115 for bioRxiv preprint. Also see Chen and Harel et al. 2024 (https://doi.org/10.1016/j.devcel.2024.04.014)
Proper citation: RRID:Addgene_166142 Copy
Species: Other
Genetic Insert: gRNA expression with modified trRNA to recruit activators via PP7 tags
Vector Backbone Description: Vector Backbone:pRDA_722; Vector Types:Lentiviral, CRISPR, Assembled Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38484704
Comments: pXPR_502 replacement. Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216106 Copy
Species: Other
Genetic Insert: P2A_v1.1; NeoR_v1.1; Stop
Vector Backbone Description: Vector Backbone:pUC57-Kan; Vector Types:Fragment; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38484704
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216066 Copy
Species: Other
Genetic Insert: P2A_v1.1; TagRFP657_v1.1; Stop
Vector Backbone Description: Vector Backbone:pUC57-Kan; Vector Types:Fragment; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38484704
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216065 Copy
Species: Streptococcus pyogenes
Genetic Insert: Cas9 [Sp]
Vector Backbone Description: Vector Backbone:pRDA_722; Vector Types:Lentiviral, CRISPR, Assembled Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38484704
Comments: pLentiCas9 replacement. Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216104 Copy
Species: Other
Genetic Insert: ITRs from AAV2
Vector Backbone Description: Vector Backbone:pRDA_889; Vector Types:Mammalian Expression, AAV, Destination; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38484704
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216080 Copy
Species: Other
Genetic Insert: for adding plate and well barcodes via 6-9 and 9-10 modules
Vector Backbone Description: Vector Backbone:pRDA_792; Vector Types:Mammalian Expression, Lentiviral, Destination; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38484704
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216084 Copy
Species: Other
Genetic Insert: gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31
Vector Backbone Description: Vector Backbone:pRDB_053; Vector Types:Insect Expression, Destination; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38484704
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216083 Copy
Species: Other
Genetic Insert: CD47 guide
Vector Backbone Description: Vector Backbone:pRDA_722; Vector Types:Lentiviral, CRISPR, Positive Controls; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38484704
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216086 Copy
Species: Other
Genetic Insert: CD47 guide
Vector Backbone Description: Vector Backbone:pRDA_722; Vector Types:Lentiviral, CRISPR, Positive Controls; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38484704
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216085 Copy
Species: Homo sapiens
Genetic Insert: FANCD2
Vector Backbone Description: Backbone Marker:Broad Institute Genetic Perturbation Platform; Backbone Size:8264; Vector Backbone:pLX_TRC317; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36608654
Comments: NM_001018115,NM_001319984,ENST00000383807,ENST00000419585 . The transcription factor ORF portion of this plasmid was synthesized by Genewiz. Please test multiple small colonies in case of plasmid recombination. To make this collection available in a timely manner, a portion of this collection was not fully sequenced by Addgene. If an Addgene verified full plasmid sequence is not available, please contact us at [email protected] prior to placing an order to request the full sequence.
Proper citation: RRID:Addgene_144382 Copy
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