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Species: Homo sapiens
Genetic Insert: HseIF4A3
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14973490
Comments: ORF extracted from NCBI:NM_014740.3 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_146581 Copy
Species: HIV
Genetic Insert: Tat
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNAI/Amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9121429
Comments: Mutant Tat gene: C30G is in the activation domain of Tat and renders Tat inactive without affecting its RNA-binding ability or protein expression levels.
Proper citation: RRID:Addgene_14656 Copy
Species: Homo sapiens
Genetic Insert: NEF NBP1
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pEF-BOS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9620685
Comments: HA tag between Nef and NBP.
Proper citation: RRID:Addgene_14657 Copy
Species: HIV
Genetic Insert: Tat
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9334327
Comments: Mutant Tat gene: K41A is in the activation domain of Tat and renders Tat inactive without affecting its RNA-binding ability or protein expression levels.
Proper citation: RRID:Addgene_14655 Copy
Species: BIV
Genetic Insert: bTat
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-KG?; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10846086
Proper citation: RRID:Addgene_14652 Copy
Species: Homo sapiens
Genetic Insert: CIITA
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pCol2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10661406
Comments: Collagen II promoter
Proper citation: RRID:Addgene_14651 Copy
Species: Caenorhabditis elegans
Genetic Insert: CeSmg5-H116E
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23348841
Comments: ORF extraced from NCBI:NM_059528.6 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_147458 Copy
Species: Homo sapiens
Genetic Insert: Keratin 18
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMRB101; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7523419
Comments: Cloning information may not be accurate. Backbone could be pcDNA3.1. The plasmid was used for transfection by the Omary lab. For example, see Ku et al 2002 JBC 277:10775, Ku et al 1998 EMBO 17:1892.
Proper citation: RRID:Addgene_14741 Copy
Species: Drosophila melanogaster
Genetic Insert: DmPAN3_349-790-P652G
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23932717
Comments: ORF extracted from NCBI: LD14901 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_147570 Copy
Species: Homo sapiens
Genetic Insert: HsTNRC6C_1281-1665
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: sequence of Hs TNRC6C isoform2 given by NCBI: NM_018996.3. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_147180 Copy
Vector Backbone Description: Backbone Size:8174; Vector Backbone:pLKO.3G; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Comments: A variant of pLKO.1 containing an eGFP marker rather than puromycin. Please note that the AgeI site is NOT unique for this vector, so shRNAs should be designed for cloning with EcoRI and PacI instead. Forward oligo: 5'-AATT—21bp sense—CTCGAG—21bp antisense—TTTTTTTAT-3'. Reverse oligo: 5'-AAAAAAA—21bp sense—CTCGAG—21bp antisense-3'.
pLKO.3G was cloned by excising PAC-GFP from pLKO.3pgw and replacing it with eGFP from another vector using the enzymes BamHI and BsRGI.
Proper citation: RRID:Addgene_14748 Copy
Species: Homo sapiens
Genetic Insert: HsNot2_344-540
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24121232
Comments: ORF extracted from NCBI: NM_014515.5. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_147618 Copy
Genetic Insert: membrane bound form of EGFP
Vector Backbone Description: Backbone Size:6122; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17209010
Comments: mGFP is a membrane-bound form of GFP containing a palmitoylation sequence of GAP43 at its N-terminus.
The mGFP insert contains V163A and S175G compared to EGFP. These mutations are not known to affect plasmid function. The Cepko lab and several other Addgene users have used this plasmid successfully in experiments.
Addgene's NGS finds an IS4-like element (ISVsa5 family transposase) in the backbone that does not affect the overall plasmid function.
Proper citation: RRID:Addgene_14757 Copy
Species: Homo sapiens
Genetic Insert: GSK3 beta K85A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8382613
Comments: Nru I site is added to the K->A mutation
Proper citation: RRID:Addgene_14755 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Flpe-ERT2 fusion protein
Vector Backbone Description: Backbone Size:4779; Vector Backbone:pCAGEN; Vector Types:Mammalian Expression, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17209010
Comments: Kozak consensus sequence was added before the start ATG. Internal EcoRI site was destroyed by introducing a silent mutation.
Proper citation: RRID:Addgene_14756 Copy
Species: Homo sapiens
Genetic Insert: HsNot2-F502A
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24121232
Comments: ORF extracted from NCBI: NM_014515.5. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_147501 Copy
Species: Homo sapiens
Genetic Insert: GSK3 beta S9A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7980435
Comments: S2A in the sequence does not affect function, used to introduce restriction enzyme site
Proper citation: RRID:Addgene_14754 Copy
Species: N. pharaonis
Genetic Insert: Mammalian codon-optimized halorhodopsin, from Natronomas pharaonis (N. pharaonis), fused with GFP at the C-terminus
Vector Backbone Description: Backbone Marker:Pavel Osten; Backbone Size:9500; Vector Backbone:FCK(1.3)GW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17375185
Comments: PLEASE CONTACT ED BOYDEN ([email protected]) FOR DETAILS ON THIS
REAGENT AND FURTHER REAGENTS IN THIS LINE.
Proper citation: RRID:Addgene_14750 Copy
Species: Homo sapiens
Genetic Insert: N-ras
Vector Backbone Description: Backbone Size:7500; Vector Backbone:pCGN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11305105
Proper citation: RRID:Addgene_14724 Copy
Species: Homo sapiens
Genetic Insert: N-ras
Vector Backbone Description: Backbone Size:7500; Vector Backbone:pCGN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11305105
Proper citation: RRID:Addgene_14725 Copy
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