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Species: E. coli
Genetic Insert: FIS
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23893733
Proper citation: RRID:Addgene_51557 Copy
Species: E. coli
Genetic Insert: FIS
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pTXB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_51564 Copy
Species: E. coli
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_53032 Copy
Species: E. coli
Genetic Insert: RhyB sRNA
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21189298
Proper citation: RRID:Addgene_52999 Copy
Species: E. coli
Genetic Insert: RhyB sRNA
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21189298
Proper citation: RRID:Addgene_53001 Copy
Species: E. coli
Genetic Insert: OxyS sRNA
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21189298
Proper citation: RRID:Addgene_53006 Copy
Species: E. coli
Genetic Insert: fimE
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_53015 Copy
Species: E. coli
Genetic Insert: etpBA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBAD/myc-His A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19707189
Comments: Please note that there is a minor mutation in etpB - E64G. This mutation should not affect function.
Proper citation: RRID:Addgene_53532 Copy
Species: E. coli
Genetic Insert: etpC
Vector Backbone Description: Backbone Size:3719; Vector Backbone:pACYC184; Vector Types:Bacterial Expression, pACYC184; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:19707189
Comments: Please note that there is a L to M mutation in etpC. This mutation should not affect function.
Proper citation: RRID:Addgene_53533 Copy
Species: e. coli
Genetic Insert: pLux
Vector Backbone Description: Backbone Size:1000; Vector Backbone:pLuxR; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24104480
Proper citation: RRID:Addgene_53544 Copy
Species: E. coli
Genetic Insert: αNTD
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9121589
Comments: This plasmid is used in conjuction with the other Ann Hochschild Bacterial 2-hybrid system plasmids (Addgene plasmids 53730, 53731, 53732, 53733, and 53734) and must be used in the reporter strain, FW102 OL2–62 (Addgene item 53735). Please see the associated article for links to all of these plasmids:
http://www.addgene.org/browse/article/8393/
The following articles can provide more information on using the bacterial 2-hybrid system:
Dove, et al. 1997 PMID 9121589
Dove and Hochschild 1998 PMID 9499408
Deaconescu, et al. 2006 PMID 16469698
Deighan, et al. 2008 PMID 18832144
Kuznedelov, et al. 2002 PMID 11823642
Proper citation: RRID:Addgene_53731 Copy
Species: E. coli
Genetic Insert: ltDHFR(DD)
Vector Backbone Description: Backbone Marker:Garry Nolan ; Backbone Size:6943; Vector Backbone:pBMN; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23991108
Comments: This N-terminal ltDHFR(DD) plasmid is the clone#22 in Table 1
Proper citation: RRID:Addgene_47076 Copy
Species: E. coli
Genetic Insert: Recoded E. coli genome with all instances of the UAG codon and RF1 removed (UAG termination function removed)
Vector Backbone Description: Vector Backbone:E. coli genome; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24136966
Comments: E. coli MG1655 Delta(ybhB-bioAB)::[lcI857 N(cro-ea59)::tetR-bla] Delta prfA Delta mutS::zeoR; all 321 UAG codons changed to UAA
This strain is mutS-, which causes a spontaneous mutation frequency of ~2E-8. This strain is auxotrophic for d-biotin.
A mutS+ version of this strain will be deposited with addgene soon.
Genome sequence deposited at NCBI (accession # CP006698)
Proper citation: RRID:Addgene_48998 Copy
Species: E. coli
Genetic Insert: tdNfsB(F124W)-mCherry
Vector Backbone Description: Backbone Marker:Scott Sternson; Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30111097
Comments: Sternson Lab recommendations for propagation of AAV-FLEX plasmids: "These vectors are prone to recombination. This is a well known issue with these AAV vectors and is due to the inverted terminal repeats (ITRs) required for AAV production. To minimize recombination, we propagate these plasmids in NEB Stable cells. Also, to minimize recombination, cells should be cultured at 30 C.
Note that these cultures will grow slowly (20 h for minipreps). Better yields and culture times are obtained with 2xYT as the media. This is strongly recommended.
Because recombination may still happen occasionally, we do a panel of restriction digestions to assess whether the ITRs are in tact. Separate digestions with PvuII, Sma1, and SnaB1 should be performed."
Proper citation: RRID:Addgene_113762 Copy
Species: E. coli
Genetic Insert: clbO
Vector Backbone Description: Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26890481
Proper citation: RRID:Addgene_114153 Copy
Species: E. coli
Genetic Insert: clbK
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26890481
Proper citation: RRID:Addgene_114152 Copy
Species: E. coli
Genetic Insert: clbH
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28805802
Proper citation: RRID:Addgene_114157 Copy
Species: E. coli
Genetic Insert: clbB
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28805802
Proper citation: RRID:Addgene_114155 Copy
Species: E. coli
Genetic Insert: ClbD
Vector Backbone Description: Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26890481
Proper citation: RRID:Addgene_114146 Copy
Species: E. coli
Genetic Insert: ClbF
Vector Backbone Description: Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26890481
Proper citation: RRID:Addgene_114148 Copy
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