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Species: Caenorhabditis elegans
Genetic Insert: spe-11 promoter (no ATG) in pDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21385 Copy
Species: Caenorhabditis elegans
Genetic Insert: pie-1 promoter (short, no ATG) in pDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21384 Copy
Species: Caenorhabditis elegans
Genetic Insert: gld-1 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21389 Copy
Species: Caenorhabditis elegans
Genetic Insert: msp-56 promoter in pDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21383 Copy
Species: Caenorhabditis elegans
Genetic Insert: him-3 prom in PDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:PDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21382 Copy
Species: Caenorhabditis elegans
Genetic Insert: lip-1 M1M2 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21381 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-119
Vector Backbone Description: Backbone Marker:Epicentre; Backbone Size:300; Vector Backbone:R6K origin PCR fragment; Vector Types:Worm Expression, Adds unc-119 marker to Amp resistant plasmids; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19520111
Comments: This plasmid has small deletions within the KanR gene. We generated these fragments by PCR so it is definitely possible that these were generated during amplification, these changes must not impair function as the construct is Kan resistant both as a plasmid and following recombination.
Proper citation: RRID:Addgene_21595 Copy
Species: Caenorhabditis elegans
Genetic Insert: (Gly)5Ala::citrine(w introns)::tbb-2 3'UTR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2639; Vector Backbone:pDONR P2R-P3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_21511 Copy
Species: Caenorhabditis elegans
Genetic Insert: (Gly)5Ala::GFP(w introns)::tbb-2 3'UTR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2639; Vector Backbone:pDONR P2R-P3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_21509 Copy
Species: Caenorhabditis elegans
Genetic Insert: BiP
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pPD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11780124
Comments: This reporter gene tracks the expression of endogenous BiP (hsp-4) in C. elegans. It consists of a ~1.1 KB genomic C. elegans fragment linked to GFP in the vector pPD95.75. The fusion between the GFP artificial gene and hsp-4 takes place at AA #3 of HSP-4)
Proper citation: RRID:Addgene_21896 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmex-5::mCherry (w introns w/o stop)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2646; Vector Backbone:pDONRP4P1R; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_22513 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-119
Vector Backbone Description: Backbone Marker:Epicentre; Backbone Size:2100; Vector Backbone:pMOD4; Vector Types:Recombineering; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19116030
Comments: Few mismatches between Addgene and depositor sequence are in the 3' UTR and shouldn't have any impact on plasmid's function
Proper citation: RRID:Addgene_19185 Copy
Species: Caenorhabditis elegans
Genetic Insert: mes-3 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19371 Copy
Species: Caenorhabditis elegans
Genetic Insert: puf-5 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19372 Copy
Species: Caenorhabditis elegans
Genetic Insert: daz-1 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19368 Copy
Species: Caenorhabditis elegans
Genetic Insert: msp-142 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19367 Copy
Species: Caenorhabditis elegans
Genetic Insert: spn-4 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19369 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi5605 targeting region
Vector Backbone Description: Backbone Size:2334; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18953339
Comments: Standard Multiple Cloning site vector to generate MosSCI inserts at ttTi5605 location. Contains C.Briggsae unc-119 rescue gene. Also contains genomic regions flanking the ttTi5605 Mos1 insertion. Use C.elegans strain EG4322 to generate insertions.
Proper citation: RRID:Addgene_19330 Copy
Species: Caenorhabditis elegans
Genetic Insert: Phsp-16::Mos1 transposase
Vector Backbone Description: Backbone Size:2530; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18953339
Comments: This plasmid was made by Dr. Jean Louis Bessereau and described in the publication: Bessereau JL, Wright A, Williams DC, Schuske K, Davis MW, Jorgensen EM.
Mobilization of a Drosophila transposon in the Caenorhabditis elegans germ line.
Nature. 2001 Sep 6;413(6851):70-4.
There is che-1 sequence in the plasmid. It comes from the glh-2 3' UTR fragment in the plasmid. The UTR fragment extends into the 3' region of the che-1 gene, which is tail to tail with glh-2 on the chromosome. This is only the last exon of che-1 and it is not relevant to the function of the construct.
Proper citation: RRID:Addgene_19333 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-3::twk-18(cn110)cDNA
Vector Backbone Description: Backbone Size:2446; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18953339
Comments: Use as co-injection marker. Negative selection marker expressed in the body wall muscles. Animals (almost) normal at 15C, paralyzed at 25C.
Proper citation: RRID:Addgene_19326 Copy
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