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Species: Sulfitobacter sp. JL08, NZ_CP025815
Genetic Insert: DinG HNH Cascade with His14-tagged Csf3
Vector Backbone Description: Vector Backbone:pET45b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37995242
Proper citation: RRID:Addgene_205963 Copy
Species: Drosophila melanogaster
Genetic Insert: HyperADARcd
Vector Backbone Description: Vector Backbone:pJG054; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34426573
Comments: Genbank: MW026677
Proper citation: RRID:Addgene_202575 Copy
Species: Homo sapiens
Genetic Insert: PML (isoform IVa)
Vector Backbone Description: Vector Backbone:Derived from Addgene ID# 52962 (Zhang Lab).; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34795231
Comments: For performing SUMO-ID or UbL-ID experiments; PMLiva is excisable using EcoR1-Not1.
Proper citation: RRID:Addgene_208038 Copy
Species: Homo sapiens
Genetic Insert: SUMO1nc
Vector Backbone Description: Backbone Marker:Open Biosystems; Vector Backbone:TRIPZ; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37996419
Comments: Lentiviral packaging of TRIPZ is more efficient with TAT expression (e.g using pcDNA1-TatHIV Addgene ID: 138478) and 2nd generation gag/pol/env combination (e.g. using PSPAX2 Addgene ID: 12260 and pMD2.G Addgene ID: 12259); Use of bioGEF-UBwt may be necessary for certain E3 ligases (e.g. NEDD4 HECT ligase); Use of "non-cleavable" versions may reduce recycling of biotinylated UB and may lead to higher yields of biotinylated protein for proteomic analysis, but induction times should be minimized to avoid toxicity.
Proper citation: RRID:Addgene_208042 Copy
Species: Homo sapiens
Genetic Insert: SUMO2nc
Vector Backbone Description: Backbone Marker:Open Biosystems; Vector Backbone:TRIPZ; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37996419
Comments: Lentiviral packaging of TRIPZ is more efficient with TAT expression (e.g using pcDNA1-TatHIV Addgene ID: 138478) and 2nd generation gag/pol/env combination (e.g. using PSPAX2 Addgene ID: 12260 and pMD2.G Addgene ID: 12259); Use of bioGEF-UBwt may be necessary for certain E3 ligases (e.g. NEDD4 HECT ligase); Use of "non-cleavable" versions may reduce recycling of biotinylated UB and may lead to higher yields of biotinylated protein for proteomic analysis, but induction times should be minimized to avoid toxicity.
Proper citation: RRID:Addgene_208043 Copy
Species: Homo sapiens
Genetic Insert: Ub
Vector Backbone Description: Backbone Marker:Open Biosystems; Vector Backbone:TRIPZ; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37996419
Comments: Lentiviral packaging of TRIPZ is more efficient with TAT expression (e.g using pcDNA1-TatHIV Addgene ID: 138478) and 2nd generation gag/pol/env combination (e.g. using PSPAX2 Addgene ID: 12260 and pMD2.G Addgene ID: 12259); Use of bioGEF-UBwt may be necessary for certain E3 ligases (e.g. NEDD4 HECT ligase); Use of "non-cleavable" versions may reduce recycling of biotinylated UB and may lead to higher yields of biotinylated protein for proteomic analysis, but induction times should be minimized to avoid toxicity.
Proper citation: RRID:Addgene_208045 Copy
Species: Candidatus Cloacimonetes bacterium (MAG), MWEE01000013
Genetic Insert: Cas8/Cse1 from MWEE01000013, Cas5-HNH
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37995242
Proper citation: RRID:Addgene_205977 Copy
Species: Arabidopsis thaliana
Genetic Insert: pU6-AarI-scaffold-term
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37682951
Proper citation: RRID:Addgene_207917 Copy
Species: Selenomonas sp. isolate RGIG9219 Water_deer_Rumen (MAG), JAGBLH010000220
Genetic Insert: Cas5 from JAGBLH010000220, Cas8-HNH
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37995242
Proper citation: RRID:Addgene_205975 Copy
Species: Candidatus Cloacimonetes bacterium (MAG), MWEE01000013
Genetic Insert: Cas7 from MWEE01000013, Cas5-HNH
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37995242
Proper citation: RRID:Addgene_205979 Copy
Species: Photinus pyralis
Genetic Insert: Firefly Luciferase
Vector Backbone Description: Backbone Size:6685; Vector Backbone:pRRLSIN.cPPT.MND; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38001268
Proper citation: RRID:Addgene_174721 Copy
Species: Homo sapiens
Genetic Insert: ACTB Homology Arms flanking a Blast-mScarlet Cassette
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39668248
Comments: To be co-transfected with sgRNA plasmid px330-PITCh-ACTB https://www.addgene.org/207748 . Please visit https://www.biorxiv.org/content/10.1101/2023.11.01.565029v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_207753 Copy
Species: Photinus pyralis
Genetic Insert: Firefly Luciferase
Vector Backbone Description: Backbone Size:6685; Vector Backbone:pRRLSIN.cPPT.MND; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38001268
Proper citation: RRID:Addgene_174722 Copy
Species: Homo sapiens
Genetic Insert: RNF4
Vector Backbone Description: Vector Backbone:Derived from Addgene ID# 52962 (Zhang Lab).; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37996419
Comments: For performing BioE3 experiments; can be used for transient expression or packaged into lentiviral particles for stable cell line selection. RNF4wt is excisable using EcoR1-Not1.
Proper citation: RRID:Addgene_208046 Copy
Species: Homo sapiens
Genetic Insert: RNF4
Vector Backbone Description: Vector Backbone:Derived from Addgene ID# 52962 (Zhang Lab).; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37996419
Comments: Control for RNF4 BioE3 experiments; contains mutations in the conserved RING domain. RNF4ca is excisable using EcoR1-Not1.
Proper citation: RRID:Addgene_208047 Copy
Species: Drosophila melanogaster
Genetic Insert: HyperADARcd
Vector Backbone Description: Vector Backbone:pER8; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34426573
Comments: Genbank: MW026674
Proper citation: RRID:Addgene_202583 Copy
Species: Selenomonas sp. isolate RGIG9219 Water_deer_Rumen (MAG), JAGBLH010000220
Genetic Insert: Cas6/Csy4 from JAGBLH010000220, Cas8-HNH
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37995242
Proper citation: RRID:Addgene_205973 Copy
Species: Synthetic
Genetic Insert: BirAopt
Vector Backbone Description: Vector Backbone:Derived from Addgene ID# 52962 (Zhang Lab).; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37996419
Comments: Expresses optimized BirA only (with short C-terminal fusion derived from GSQ linker, RBXN polylinker, and P2A remnant). For performing BioE3 experiments; can be used for transient expression or packaged into lentiviral particles for stable cell line selection.
Proper citation: RRID:Addgene_208048 Copy
Species: Candidatus Cloacimonetes bacterium (MAG), MWEE01000013
Genetic Insert: Cas5-HNH crRNA
Vector Backbone Description: Vector Backbone:pColADuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37995242
Proper citation: RRID:Addgene_205971 Copy
Species: Sars Cov 2
Genetic Insert: Sars Cov 2 MPRO
Vector Backbone Description: Vector Backbone:pET28-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: https://asapdiscovery.org/outputs/target-enabling-packages/
Proper citation: RRID:Addgene_204770 Copy
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