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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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|---|---|---|---|---|---|---|---|---|---|---|
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p663-UBC-ER-TurboID-V5_IDG-K Resource Report Resource Website |
RRID:Addgene_214453 | ER-TurboID-V5 | Other | Ampicillin | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2026-09-12 02:40:20 | 0 | ||
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p663-UBC-PM-miniTurbo-V5_IDG-K Resource Report Resource Website |
RRID:Addgene_214454 | PM-miniTurbo-V5 | Other | Ampicillin | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2026-09-12 02:40:20 | 0 | ||
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eNme2C-CBE6d Resource Report Resource Website |
RRID:Addgene_215832 | CBE6d-eNme2C-2xUGI | Ampicillin | PMID:38402281 | Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:40:19 | 0 | |||
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SpCas9-CBE6b-IVT Resource Report Resource Website 1+ mentions |
RRID:Addgene_215834 | CBE6b-SpCas9-2xUGI | Ampicillin | PMID:38402281 | Vector Backbone:pCMV with a BR322 origin; Vector Types:in vitro Transcription Template; Bacterial Resistance:Ampicillin | 2026-09-12 02:40:19 | 1 | |||
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SpCas9-CBE6a-IVT Resource Report Resource Website |
RRID:Addgene_215833 | CBE6a-SpCas9-2xUGI | Ampicillin | PMID:38402281 | Vector Backbone:pCMV with a BR322 origin; Vector Types:in vitro Transcription Template; Bacterial Resistance:Ampicillin | 2026-09-12 02:40:19 | 0 | |||
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p663-UBC-miniTurbo-V5-CSNK1G3_IDG-K Resource Report Resource Website |
RRID:Addgene_214460 | miniTurbo-V5-CSNK1G3 | Homo sapiens | Ampicillin | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2026-09-12 02:40:20 | 0 | ||
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p663-UBC-miniTurbo-V5-PRPF4B_IDG-K Resource Report Resource Website |
RRID:Addgene_214463 | miniTurbo-V5-PRPF4B | Homo sapiens | Ampicillin | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2026-09-12 02:40:20 | 0 | ||
|
eNme2C-CBE6b Resource Report Resource Website |
RRID:Addgene_215830 | CBE6b-eNme2C-2xUGI | Ampicillin | PMID:38402281 | Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:40:19 | 0 | |||
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p667-UBC-STK17A-V5-miniTurbo_IDG-K Resource Report Resource Website |
RRID:Addgene_214466 | STK17A-V5-miniTurbo | Homo sapiens | Ampicillin | These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. | Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin | 2026-09-12 02:40:20 | 0 | ||
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pLKO-Tet-On-ARNT-shRNA1 Resource Report Resource Website |
RRID:Addgene_166910 | ARNT shRNA | Homo sapiens | Ampicillin | PMID:37620297 | Backbone Size:11000; Vector Backbone:pLKO-TET-ON-addgene#21915; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-12 02:40:19 | 0 | ||
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pCRISPR-cBEST-v2-kasOP* Resource Report Resource Website |
RRID:Addgene_209447 | Codon optimized APOBEC1-nCas9-UGI fusion protein | Synthetic | Apramycin | PMID:38340355 | Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin | 2026-09-12 02:40:19 | 0 | ||
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EF1a-SRRD-noTag (in pLEX_307) Resource Report Resource Website |
RRID:Addgene_214919 | SRRD | Homo sapiens | Ampicillin | PMID:38315730 | Vector Backbone:pLEX_307; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-12 02:40:19 | 0 | ||
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pCRISPR-cBEST-v2-PermE Resource Report Resource Website |
RRID:Addgene_209446 | Codon optimized APOBEC1-nCas9-UGI fusion protein | Synthetic | Apramycin | PMID:38340355 | Vector Backbone:pGM1190; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Apramycin | 2026-09-12 02:40:19 | 0 | ||
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pSWEET-bgaB Resource Report Resource Website |
RRID:Addgene_210272 | bgaB | B. stearothermophilus | Ampicillin | PMID:11133472 | Vector Backbone:pSWEET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-09-12 02:40:19 | 0 | ||
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pLKO-Tet-On-ARNTL-shRNA2 Resource Report Resource Website |
RRID:Addgene_166914 | ARNTL shRNA | Homo sapiens | Ampicillin | PMID:37620297 | Backbone Size:11000; Vector Backbone:pLKO-TET-ON-addgene#21915; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-12 02:40:19 | 0 | ||
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pLP-pTac-himar-apraR Resource Report Resource Website |
RRID:Addgene_199995 | Landing Pads | Synthetic | Ampicillin and Spectinomycin | PMID:35366417 | This material contains 2 plasmids. The second plasmid, p15A-spec-inh1, is designed to inhibit the activity of transposase or integrase in the primary plasmid, pPath-pTac-integrase. | Vector Backbone:Custom; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Spectinomycin | himar frameshift | 2026-09-12 02:40:19 | 0 |
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pLKO-Tet-On-ARNTL-shRNA3 Resource Report Resource Website |
RRID:Addgene_166915 | ARNTL shRNA | Homo sapiens | Ampicillin | PMID:37620297 | Backbone Size:11000; Vector Backbone:pLKO-TET-ON-addgene#21915; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-12 02:40:19 | 0 | ||
|
TRE-SRRD-mRuby3 Resource Report Resource Website |
RRID:Addgene_214920 | SRRD | Homo sapiens | Ampicillin | PMID:38315730 | Vector Backbone:lab generated; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-12 02:40:19 | 0 | ||
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EF1a-SRRD-V5-APEX2 (in pLEX_307) Resource Report Resource Website |
RRID:Addgene_214921 | SRRD | Homo sapiens | Ampicillin | PMID:38315730 | Vector Backbone:pLEX_307; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-12 02:40:19 | 0 | ||
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TRE-mRuby3 Resource Report Resource Website |
RRID:Addgene_214923 | mRuby3 | Synthetic | Ampicillin | PMID:38315730 | Vector Backbone:lab generated; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-09-12 02:40:19 | 0 |
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