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Genetic Insert: int Giles; gfpm2+; xylEm; ttsbiA, ttsbiB
Vector Backbone Description: Vector Backbone:pML603; Vector Types:bacterial integration vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20692326
Comments: This is an improved Giles integration vector for stable gene integration in mycobacteria with codon optimized gfp and xylE as reporter genes; terminators to protect expression cassette against transcriptional
interference.
Proper citation: RRID:Addgene_32378 Copy
Species: Homo sapiens
Genetic Insert: SMC1A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pCDNA3 cMyc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11877376
Proper citation: RRID:Addgene_32371 Copy
Species: Homo sapiens
Genetic Insert: SMC1A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pCDNA3 cMyc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11877376
Proper citation: RRID:Addgene_32372 Copy
Species: Mus musculus
Genetic Insert: Mdm2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3Basic; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15090541
Proper citation: RRID:Addgene_32370 Copy
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P5-P2; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21772812
Proper citation: RRID:Addgene_32416 Copy
Genetic Insert: EYFP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221 L5-L4; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21772812
Proper citation: RRID:Addgene_32417 Copy
Genetic Insert: oPRE
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P5-P2; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21772812
Comments: Gene Therapy (2006) 13, 641–645. doi:10.1038/sj.gt.3302698; published online 15 December 2005
'Woodchuck hepatitis virus post-transcriptional regulatory element deleted from X protein and promoter sequences enhances retroviral vector titer and expression. Schambach A, Bohne J, Baum C, Hermann FG, Egerer L, von Laer D, Giroglou T. Gene Ther. 2006 Apr;13(7):641-5.'
Addgene Sanger sequencing confirms that all four ATGs in the oPRE have been mutated to TAGs
Proper citation: RRID:Addgene_32414 Copy
Species: Mus musculus
Genetic Insert: Mdm2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3Basic; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15090541
Proper citation: RRID:Addgene_32365 Copy
Species: Homo sapiens
Genetic Insert: SMC1A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pCDNA3 cMyc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11877376
Proper citation: RRID:Addgene_32363 Copy
Genetic Insert: nestin promoter/enhancer GFP
Vector Backbone Description: Vector Backbone:CX lentiviral vector; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16713999
Comments: Note that there are some discrepancies between Addgene's QC sequence and the depositor's assembled sequence. The plasmid should still function as reported.
Proper citation: RRID:Addgene_32401 Copy
Vector Backbone Description: Backbone Marker:Mark Bushell; Backbone Size:6000; Vector Backbone:pEMCS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18296487
Comments: Please note that Addgene's sequencing result found a 60 nucleotide insert between bp#750/751 when compared to the full plasmid sequence provided by the depositing scientist. The depositing scientist confirmed that Addgene's sequence is correct--the insertion is located downstream of the CD4 bio coding sequence and is not a concern for protein expression.
Proper citation: RRID:Addgene_32402 Copy
Genetic Insert: ColE1 origin replication
Vector Backbone Description: Vector Backbone:pMS2Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11707328
Comments: This is a mycobacterial shuttle vector with a lower copy number in E. coli.
Proper citation: RRID:Addgene_32360 Copy
Genetic Insert: pJAZ38 ori
Vector Backbone Description: Vector Backbone:pMS2; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:11707328
Comments: This is a low copy number (one?) plasmid that co-replicates with pAL5000-derived plasmids in mycobacteria.
Proper citation: RRID:Addgene_32361 Copy
Vector Backbone Description: Backbone Marker:Mark Bushell; Backbone Size:6000; Vector Backbone:pEMCS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18296487
Comments: Please note that Addgene's sequencing result with the rCD4-F primer found a single nucleotide mismatch at bp# 858, when compared to the full plasmid sequence provided by the depositing scientist. According to the depositing scientist, this is a silent mutation in the COMP domain of the protein and should not affect protein expression.
Proper citation: RRID:Addgene_32403 Copy
Species: Mus musculus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21669867
Comments: A W374C mutation is also present in the pEGFP-N1-CaMKKbeta constructs that were used in this publication.
Proper citation: RRID:Addgene_32453 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18460329
Comments: The S3V, G96R, and P108L "mutations" were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations.
Proper citation: RRID:Addgene_32451 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21669867
Comments: The S3V, G96R, and P108L "mutations" were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations.
Proper citation: RRID:Addgene_32456 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21669867
Comments: The S3V, G96R, and P108L "mutations" were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations.
Proper citation: RRID:Addgene_32457 Copy
Genetic Insert: eGFP
Vector Backbone Description: Backbone Size:2374; Vector Backbone:pUCBB; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22033566
Proper citation: RRID:Addgene_32562 Copy
Genetic Insert: G-GECO1.1
Vector Backbone Description: Backbone Size:3200; Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21903779
Comments: Note: Could not make stable cell line using this vector.
Proper citation: RRID:Addgene_32445 Copy
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