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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pJCSUP35(1-122)
 
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RRID:Addgene_1091 SUP35 Saccharomyces cerevisiae Ampicillin PMID:9182769 Backbone Marker:J. Clos and Brandau, 1994; Backbone Size:3000; Vector Backbone:pJC45; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin amino acids 1 - 122 of SUP35 2026-09-12 02:14:49 0
pcDNA3-E2F4
 
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1+ mentions
RRID:Addgene_10914 E2F4 Homo sapiens Ampicillin PMID:7892279 See author's map. Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-12 02:14:49 6
pcDNA type III TGF beta receptor (R3-OFF)
 
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RRID:Addgene_10913 R3-OFF Rattus norvegicus Kanamycin PMID:1657407 Backbone Size:7100; Vector Backbone:pcDNAI neo; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-09-12 02:14:49 0
pBabe-Dvl2-GFP
 
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RRID:Addgene_109148 Dvl2 Homo sapiens Ampicillin PMID:27744318 Gene Origin - PMID: 16263761 Backbone Marker:Addgene #1764; Backbone Size:5169; Vector Backbone:pBabe-Puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-09-12 02:14:49 0
Rc/CMV cyclin B2
 
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1+ mentions
RRID:Addgene_10911 cyclin B2 Homo sapiens Ampicillin PMID:1388095 See author's map. Backbone Size:5542; Vector Backbone:pRc/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-12 02:14:49 2
NK590
 
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RRID:Addgene_109084 mPapaya Synthetic Ampicillin PMID:30281390 pDSB_0031 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint. Vector Backbone:pDSB_0029; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin 2026-09-12 02:14:49 0
NK594
 
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RRID:Addgene_109085 TagRFP-T Synthetic Ampicillin PMID:30281390 pDSB_0035 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint. Vector Backbone:pDSB_0029; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin 2026-09-12 02:14:49 0
NK597
 
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RRID:Addgene_109086 tdTomato Synthetic Ampicillin PMID:30281390 pDSB_0040 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint. Vector Backbone:pDSB_0029; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin 2026-09-12 02:14:49 0
NK610
 
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RRID:Addgene_109087 mCherry Synthetic Ampicillin PMID:30281390 pDSB_0056 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint. Vector Backbone:pDSB_0011; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin 2026-09-12 02:14:49 0
NK611
 
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RRID:Addgene_109088 mCherry Synthetic Ampicillin PMID:30281390 pDSB_0057 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint. Vector Backbone:pDSB_0029; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin 2026-09-12 02:14:49 0
NK591
 
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RRID:Addgene_109080 mTFP1 Synthetic Ampicillin PMID:30281390 pDSB_0032 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint. Vector Backbone:pDSB_0029; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin 2026-09-12 02:14:49 0
pHS15 (hANGPTL3-Strep)
 
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RRID:Addgene_109112 ANGPTL3 Homo sapiens Ampicillin PMID:29031715 21bp deletion in the SV40 promoter sequence. May affect blasticidin selection. Backbone Size:4989; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-12 02:14:49 0
pEB30 (hANGPTL8-V5-His)
 
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RRID:Addgene_109114 ANGPTL8 Homo sapiens Ampicillin PMID:29031715 Backbone Size:5058; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-12 02:14:49 0
pEB31 (hANGPTL8-V5-His lenti)
 
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RRID:Addgene_109116 ANGPTL8 Homo sapiens Ampicillin PMID:29031715 Backbone Size:8031; Vector Backbone:pRRL-cPPT-MCS-IRES-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-09-12 02:14:49 0
mito-gTEMP_pcDNA3
 
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1+ mentions
RRID:Addgene_109117 mito-gTEMP Synthetic Ampicillin PMID:28212432 Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-12 02:14:49 2
nuc-gTEMP_pcDNA3
 
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RRID:Addgene_109118 nuc-gTEMP Synthetic Ampicillin PMID:28212432 Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-12 02:14:49 0
AICSDP-47: CTNNB1-mEGFP
 
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RRID:Addgene_109119 CTNNB1 Homology Arms with mEGFP-linker Homo sapiens Ampicillin This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the N-terminus of human CTNNB1 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: SGLRSRAQASNSAVDGTAAT. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin homology arms contain point mutations to disrupt crRNA binding sites used 2026-09-12 02:14:49 0
NK644
 
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1+ mentions
RRID:Addgene_109096 PTSG_00306 geranyl-geranylation signal Synthetic Ampicillin PMID:30281390 pDSB_0104 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint. Vector Backbone:pDSB_0057; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin 2026-09-12 02:14:49 2
NK633
 
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1+ mentions
RRID:Addgene_109097 mTFP1_3'UTR Act Synthetic Ampicillin PMID:30281390 pDSB_0093 . Please visit https://www.biorxiv.org/content/early/2018/06/09/343111 for bioRxiv preprint. Vector Backbone:pDSB_0050; Vector Types:Choanoflagellate Expression; Bacterial Resistance:Ampicillin 2026-09-12 02:14:49 1
pCAG-eiC++-mRuby2-ST
 
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RRID:Addgene_109132 eiC++-mRuby2-ST Ampicillin PMID:29713079 Backbone Size:5833; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-09-12 02:14:49 0

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