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Species: Homo sapiens
Genetic Insert: MEK
Vector Backbone Description: Backbone Size:2503; Vector Backbone:pME; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19269286
Comments: Middle entry clone.
Proper citation: RRID:Addgene_31166 Copy
Genetic Insert: chimeric fli1ep enhancer/promoter fragment
Vector Backbone Description: Backbone Size:2810; Vector Backbone:p5E; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17948311
Comments: "p5E" refers to 5' entry clones flanked by L4-R1.
Drives endothelial expression.
See publication for more details: Dev Dyn. 2007 Nov . 236(11):3077-87 PMID: 17948311 https://www.ncbi.nlm.nih.gov/pubmed/17948311
Proper citation: RRID:Addgene_31160 Copy
Species: Xanthamonas oryzae
Genetic Insert: repeat NK6
Vector Backbone Description: Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Comments: IMPORTANT NOTE!: Restriction digests show that the pNK series of plasmids is actually about 2.5kb, not 3.1kb as the sequence-based map below suggests. All important features are present in the plasmid and the construct should function as reported despite this size discrepancy.
Proper citation: RRID:Addgene_31041 Copy
Species: Xanthamonas oryzae
Genetic Insert: TALE
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2676; Vector Backbone:pCR8-GW modified; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21493687
Comments: Gateway compatible vector for assembly of full-length TAL effector genes by Golden Gate cloning. Contains both SD and Kozak sequences immediately upstream of the initial ATG. Designed for TAL DNA-binding domains alone
Proper citation: RRID:Addgene_31031 Copy
Species: Homo sapiens
Genetic Insert: MITF-M
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-TAG4A; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19422606
Proper citation: RRID:Addgene_31152 Copy
Species: Xanthamonas oryzae
Genetic Insert: TALEN-FokI nuclease backbone
Vector Backbone Description: Backbone Size:5582; Vector Backbone:pDW1789; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21493687
Comments: Expression of the TAL-FokI homodimer in yeast.
Proper citation: RRID:Addgene_31034 Copy
Species: Homo sapiens
Genetic Insert: MITF-M
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-TAG4A; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19422606
Proper citation: RRID:Addgene_31154 Copy
Species: Rattus norvegicus
Genetic Insert: Regulator of G Protein Signaling 4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9294233
Proper citation: RRID:Addgene_31157 Copy
Species: Xanthamonas oryzae
Genetic Insert: TALEN-FokI nuclease backbone
Vector Backbone Description: Backbone Size:6519; Vector Backbone:pDW1789_Leu; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21493687
Comments: Expression of the TAL-FokI homodimer in yeast.
NOTE: The GenBank file associated with this plasmid (accessible from the Golden Gate TALEN kit page) erroneously lists this plasmid as having a D450A codon (A--C) FokI mutation.
Proper citation: RRID:Addgene_31035 Copy
Species: Xanthamonas oryzae
Genetic Insert: repeat NK3
Vector Backbone Description: Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Comments: IMPORTANT NOTE!: Restriction digests show that the pNK series of plasmids is actually about 2.5kb, not 3.1kb as the sequence-based map below suggests. All important features are present in the plasmid and the construct should function as reported despite this size discrepancy.
Proper citation: RRID:Addgene_31038 Copy
Species: Xanthamonas oryzae
Genetic Insert: repeat NK2
Vector Backbone Description: Backbone Size:2989; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Comments: IMPORTANT NOTE!: Restriction digests show that the pNK series of plasmids is actually about 2.5kb, not 3.1kb as the sequence-based map below suggests. All important features are present in the plasmid and the construct should function as reported despite this size discrepancy.
Proper citation: RRID:Addgene_31037 Copy
Genetic Insert: basal adenovirus E1b TATA element
Vector Backbone Description: Backbone Size:2810; Vector Backbone:p5E; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Comments: "p5E" refers to 5' entry clones flanked by L4-R1
Proper citation: RRID:Addgene_31158 Copy
Genetic Insert: LacZ + BsaI restriction sites
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2447; Vector Backbone:pCR8; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_31030 Copy
Species: Homo sapiens
Genetic Insert: MITF-M
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-TAG4A; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19422606
Proper citation: RRID:Addgene_31151 Copy
Species: Homo sapiens
Genetic Insert: ADAM7
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21618342
Proper citation: RRID:Addgene_31141 Copy
Species: Homo sapiens
Genetic Insert: ADAM29
Vector Backbone Description: Backbone Marker:Systems Biosciences Inc; Backbone Size:6600; Vector Backbone:pCDF1-EF1-MCS2-Puromycin; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21618342
Proper citation: RRID:Addgene_31145 Copy
Genetic Insert: LacZ + BsaI restriction sites
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2447; Vector Backbone:pCR8; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_31027 Copy
Species: firefly luciferase from pGL3-Basic
Genetic Insert: firefly luciferase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5150; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15520459
Comments: for Flp mediated site directed integration, tetracycline inducibilty, to measure tetracycline regulation by Tet repressor
Proper citation: RRID:Addgene_31098 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FLP recombinase
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11376165
Proper citation: RRID:Addgene_31130 Copy
Species: Homo sapiens
Genetic Insert: HNF4A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5150; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Flp/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19835622
Comments: for Flp mediated site directed integration, tetracycline inducibilty by Tet operator in CMV promoter shortened to -218.
Corresponding wild type (full length) CMV promoter construct in FR_HNF4A2 (Thomas et al 2004).
Proper citation: RRID:Addgene_31090 Copy
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