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Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3457; Vector Backbone:pBluescript; Vector Types:Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27913727
Comments: The Sleeping Beauty transposon system consists of two components: the transposon vector and the transposase vector. The transposon vector (pT4/HB, Addgene #108352) contains binding sites flanking the GOI. And the transposase vector (pCMV(CAT)T7-SB100, Addgene #34879) encodes for the enzyme mediating excision and integration of the GOI.
Proper citation: RRID:Addgene_108352 Copy
Species: Homo sapiens
Genetic Insert: WNT3A, Wnt family member 3A
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRESneo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30794657
Proper citation: RRID:Addgene_108345 Copy
Species: TALEN
Genetic Insert: TALEN
Vector Backbone Description: Backbone Marker:Matthew Porteus; Backbone Size:7053; Vector Backbone:MR015; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509632
Proper citation: RRID:Addgene_108342 Copy
Species: Synthetic
Genetic Insert: UAS:[GlyRa1 secretion sequence]-[iGluSnFR]
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC19; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30078569
Comments: Cloning was via SLiCE (Seamless Ligation Cloning Extract).
Note: The right tol1 region contains a single base pair mismatch compared to the depositor's expected sequence. The depositor has noted that this could reduce transgenesis efficiency, although they were able to successfully establish lines using the parent vector.
Proper citation: RRID:Addgene_108356 Copy
Species: Synthetic
Genetic Insert: B3 recombinase
Vector Backbone Description: Backbone Size:2720; Vector Backbone:pUC57; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30078569
Proper citation: RRID:Addgene_108354 Copy
Vector Backbone Description: Backbone Marker:Dr. Feng Zhang's Lab; Backbone Size:9175; Vector Backbone:pX459; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29672770
Proper citation: RRID:Addgene_108297 Copy
Vector Backbone Description: Backbone Marker:Dr. Feng Zhang's Lab; Backbone Size:9175; Vector Backbone:pX459; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29672770
Proper citation: RRID:Addgene_108296 Copy
Species: Synthetic
Genetic Insert: Bxb1 attB site
Vector Backbone Description: Backbone Marker:Kenan Murphy; Vector Backbone:pKM435; Vector Types:ORBIT integration plasmid for creating knockouts of target genes; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30538179
Comments: ORBIT integrating plasmid for creating knockouts of target genes.
Strain should be grown in 20 ug/ml chloramphenicol for isolation of plasmid. Yields with Qiagen miniprep kits are typically in the range from 60-90 ug/ml from 5 ml culture.
Proper citation: RRID:Addgene_108322 Copy
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29620866
Proper citation: RRID:Addgene_108336 Copy
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29620866
Proper citation: RRID:Addgene_108335 Copy
Vector Backbone Description: Backbone Marker:Dr. Feng Zhang's Lab; Backbone Size:9175; Vector Backbone:pX459; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29672770
Proper citation: RRID:Addgene_108298 Copy
Species: Caenorhabditis elegans
Genetic Insert: Streptothricin acetyl transferase 2
Vector Backbone Description: Backbone Marker:C.I. Bargmann and S. MaCarroll; Backbone Size:3500; Vector Backbone:delta pSM; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29742140
Proper citation: RRID:Addgene_108331 Copy
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29620866
Proper citation: RRID:Addgene_108339 Copy
Species: Mus musculus
Genetic Insert: Pkd1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:modified pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29426897
Proper citation: RRID:Addgene_108395 Copy
Species: Mus musculus
Genetic Insert: Pkd1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:modified pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29426897
Proper citation: RRID:Addgene_108392 Copy
Species: Mus musculus
Genetic Insert: Pkd1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:modified pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29426897
Proper citation: RRID:Addgene_108390 Copy
Species: Synthetic
Genetic Insert: RspCas13d
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5390; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29551514
Comments: For commercial use, please contact Arbor Biotechnologies at [email protected].
Proper citation: RRID:Addgene_108305 Copy
Vector Backbone Description: Backbone Marker:Dr. Feng Zhang's Lab; Backbone Size:8506; Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29672770
Proper citation: RRID:Addgene_108302 Copy
Species: Synthetic
Genetic Insert: dxCas(3.6)-VPR
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29512652
Proper citation: RRID:Addgene_108388 Copy
Species: Synthetic
Genetic Insert: xCas9(3.6)-ABE(7.10)
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29512652
Proper citation: RRID:Addgene_108387 Copy
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