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Species: Homo sapiens
Genetic Insert: Argonaute 2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5313; Vector Backbone:pIRESneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15260970
Comments: A small insertion in the 5'UTR should not affect the expressed peptide.
Proper citation: RRID:Addgene_10821 Copy
Species: Homo sapiens
Genetic Insert: Argonaute 4
Vector Backbone Description: Backbone Size:5310; Vector Backbone:pIRESneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15260970
Comments: A small insertion in the 5'UTR should not affect the expressed peptide.
Proper citation: RRID:Addgene_10824 Copy
Species: Homo sapiens
Genetic Insert: Argonaute 3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5310; Vector Backbone:pIRESneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15260970
Comments: A small insertion in the 5'UTR should not affect the expressed peptide.
Proper citation: RRID:Addgene_10823 Copy
Vector Backbone Description: Backbone Marker:Jörg Kudla; Backbone Size:16025; Vector Backbone:UBQ10:sXVE:GFPc:Kan; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29451720
Proper citation: RRID:Addgene_108260 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30478458
Comments: The three new “Marionette” strains contain the Marionette cluster, which expresses 12 inducible transcription factors, from the genome. Plasmids pAJM.711, pAJM.712, pAJM.713, pAJM.714, pAJM.715, pAJM.717, pAJM.716, pAJM.718, pAJM.719, pAJM.1459, pAJM.721 are reporters for those 12 transcription factors. For examples: TetR is being expressed from the genome of the Marionette strains, and pAJM.717 has a promoter that is repressed by TetR (PTet*) driving YFP. pAJM.717 should not produce YFP, until the cell is exposed to anhydrotetracycline (aTc). Other plasmids from our laboratory encode both the reporter and the transcription factor, and should be used in a regular (non-Marionette) strain. For example: pAJM.011 expresses TetR and contains a PTet*-YFP reporter module.
Please visit https://www.biorxiv.org/content/early/2018/04/10/285866 for bioRxiv preprint.
Proper citation: RRID:Addgene_108254 Copy
Species: Synthetic
Genetic Insert: S. solfataricus PCNA1
Vector Backbone Description: Backbone Marker:Merck Millipore; Backbone Size:5330; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29663675
Proper citation: RRID:Addgene_108197 Copy
Species: Synthetic
Genetic Insert: PdR-PCNA3(R112C/T180C) fusion protein
Vector Backbone Description: Backbone Marker:Merck Millipore; Backbone Size:5271; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30039932
Proper citation: RRID:Addgene_108195 Copy
Species: Synthetic
Genetic Insert: PdR-PCNA3(R112C/T180C)-P450cam fusion protein
Vector Backbone Description: Backbone Marker:Merck Millipore; Backbone Size:5271; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30039932
Proper citation: RRID:Addgene_108194 Copy
Species: Homo sapiens
Genetic Insert: EYFP-p23
Vector Backbone Description: Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17010336
Proper citation: RRID:Addgene_108225 Copy
Species: Homo sapiens
Genetic Insert: mCherry-p23
Vector Backbone Description: Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17010336
Proper citation: RRID:Addgene_108224 Copy
Species: Synthetic
Genetic Insert: PCNA1(G108C)-PdX fusion protein
Vector Backbone Description: Backbone Marker:Merck Millipore; Backbone Size:5697; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30039932
Proper citation: RRID:Addgene_108189 Copy
Species: Homo sapiens
Genetic Insert: EGFP-Hsp90b
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17010336
Proper citation: RRID:Addgene_108221 Copy
Species: Homo sapiens
Genetic Insert: AJUBA
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:13320; Vector Backbone:pTRIPZ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29440237
Comments: Please note that due to a cloning artifact, the amino acids SLDN were added to the C-terminus of Ajuba. The construct will function as described in the associated publication.
Proper citation: RRID:Addgene_108229 Copy
Species: Synthetic
Genetic Insert: LexA DNA binding domain fusing with anti-RNase A VHH antibody
Vector Backbone Description: Backbone Size:6363; Vector Backbone:pSB4K5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28946740
Proper citation: RRID:Addgene_108241 Copy
Species: Synthetic
Genetic Insert: LexA DNA binding domain (1-87)
Vector Backbone Description: Backbone Size:6363; Vector Backbone:pSB4K5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28946740
Proper citation: RRID:Addgene_108240 Copy
Species: Synthetic
Genetic Insert: Pc promoter - mTurquoise2
Vector Backbone Description: Backbone Marker:obtained from Culture Collection of Switzerland; Backbone Size:8248; Vector Backbone:pME6031; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_108235 Copy
Species: Homo sapiens
Genetic Insert: PKD3 RNAi
Vector Backbone Description: Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16100512
Comments: Constructed by ligation of the following oligonucleotide pairs to pSUPER: 5'-GATCCCCGTC CTAAGACGGG ACTCTCTTCA AGAGAGAGAG TCCCGTCTTA GGACTTTTTG GAAA-3' and 5'-AGCTTTTCCA AAAAGTCCTA AGACGGGACT CTCTCTCTTG AAGAGAGTCC CGTCTTAGGA CGGG-3'.
Proper citation: RRID:Addgene_10818 Copy
Species: Synthetic
Genetic Insert: S. solfataricus PCNA3
Vector Backbone Description: Backbone Marker:Merck Millipore; Backbone Size:5330; Vector Backbone:pET-28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29663675
Proper citation: RRID:Addgene_108199 Copy
Vector Backbone Description: Backbone Marker:Dr. Feng Zhang's Lab; Backbone Size:9175; Vector Backbone:pX459; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29672770
Proper citation: RRID:Addgene_108294 Copy
Species: Homo sapiens
Genetic Insert: CHMP1B
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4993; Vector Backbone:pGEX2T-TEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32251413
Proper citation: RRID:Addgene_108291 Copy
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