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Species: Synthetic
Genetic Insert: 3xHA-NLS-dSpCas9-T2A-EGFP
Vector Backbone Description: Backbone Size:4237; Vector Backbone:PX458; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38942756
Proper citation: RRID:Addgene_201953 Copy
Species: Synthetic
Genetic Insert: bpNLS-FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
Vector Backbone Description: Backbone Size:4230; Vector Backbone:PX458; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38942756
Proper citation: RRID:Addgene_201955 Copy
Species: S. stipitis (yeast)
Genetic Insert: TEF1 promoter (S. stipitis)
Vector Backbone Description: Backbone Marker:Robertson; Backbone Size:8299; Vector Backbone:pWR54; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37345937
Proper citation: RRID:Addgene_203331 Copy
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Backbone Size:4356; Vector Backbone:pUC18R6KT-mini-Tn7T-Km; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38715147
Comments: Ec100D pir-116 genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir-116(DHRF) Maintains plasmids at ~250 copies per cell.
This strain is naturally resistant to streptomycin
Proper citation: RRID:Addgene_200989 Copy
Genetic Insert: VHH_H6
Vector Backbone Description: Backbone Marker:Antibody Design Laboratories; Backbone Size:3960; Vector Backbone:pADL-23c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38835241
Proper citation: RRID:Addgene_201719 Copy
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:5262; Vector Backbone:pTn7-SCOUT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:38715147
Comments: Ec100D pir-116 genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir-116(DHRF) Maintains plasmids at ~250 copies per cell.
This strain is naturally resistant to streptomycin
Proper citation: RRID:Addgene_200992 Copy
Species: lama glama (llama)
Genetic Insert: VHH B5-5
Vector Backbone Description: Backbone Marker:Antibody Design Laboratories; Backbone Size:3960; Vector Backbone:pADL-23c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38835241
Proper citation: RRID:Addgene_201721 Copy
Species: lama glama (llama)
Genetic Insert: VHH_A10-5
Vector Backbone Description: Backbone Marker:Antibody Design Laboratories; Backbone Size:3960; Vector Backbone:pADL-23c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38835241
Proper citation: RRID:Addgene_201720 Copy
Species: S. stipitis (yeast)
Genetic Insert: TEF1 promoter (S. stipitis)
Vector Backbone Description: Backbone Marker:Robertson; Vector Backbone:pWR9; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37345937
Proper citation: RRID:Addgene_202776 Copy
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:5565; Vector Backbone:pTn7-SCOUT20; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:38715147
Comments: Ec100D pir-116 genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir-116(DHRF) Maintains plasmids at ~250 copies per cell.
This strain is naturally resistant to streptomycin
Proper citation: RRID:Addgene_200996 Copy
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:6696; Vector Backbone:pTn7-SCOUT20; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:38715147
Comments: Ec100D pir-116 genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir-116(DHRF) Maintains plasmids at ~250 copies per cell.
This strain is naturally resistant to streptomycin
Proper citation: RRID:Addgene_200997 Copy
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:5556; Vector Backbone:pTn7-SCOUT20; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Spectinomycin
Defining Citation: PMID:38715147
Comments: Ec100D pir-116 genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir-116(DHRF) Maintains plasmids at ~250 copies per cell.
This strain is naturally resistant to streptomycin
Proper citation: RRID:Addgene_200998 Copy
Species: Synthetic
Genetic Insert: pD4H-FLUC-tOCS; pNOS-RLUC-tNOS
Vector Backbone Description: Backbone Marker:Mortensen et al., 2019 (doi:10.3389/fpls.2019.00755); Vector Backbone:pSB90; Vector Types:Plant Expression, Luciferase; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_203901 Copy
Vector Backbone Description: Backbone Marker:Philip Poole lab; Backbone Size:4659; Vector Backbone:pTn7-SCOUT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38715147
Comments: Ec100D pir-116 genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir-116(DHRF) Maintains plasmids at ~250 copies per cell.
This strain is naturally resistant to streptomycin
Proper citation: RRID:Addgene_200990 Copy
Species: Mus musculus
Genetic Insert: VprBP
Vector Backbone Description: Backbone Size:8297; Vector Backbone:GFP-TDG (pc2422); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36056023
Proper citation: RRID:Addgene_201698 Copy
Species: S. stipitis (yeast)
Genetic Insert: GSC2 plus flanking
Vector Backbone Description: Backbone Marker:Robertson; Backbone Size:8072; Vector Backbone:pWR88; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37345937
Proper citation: RRID:Addgene_203914 Copy
Species: S. stipitis (yeast)
Genetic Insert: URA3 upstream homology
Vector Backbone Description: Backbone Marker:Robertson; Backbone Size:8093; Vector Backbone:pWR95; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37345937
Proper citation: RRID:Addgene_203917 Copy
Species: Synthetic
Genetic Insert: Signal peptide from E. coli PhoA protein
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_204107 Copy
Species: Homo sapiens
Genetic Insert: H2AX
Vector Backbone Description: Vector Backbone:pSpCas9(BB)-2A-Puro(PX459)V2.0(#62988); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38739603
Proper citation: RRID:Addgene_204745 Copy
Species: Homo sapiens
Genetic Insert: H2AX K5Q
Vector Backbone Description: Vector Backbone:pSpCas9(BB)-2A-Puro(PX459)V2.0(#62988); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38739603
Proper citation: RRID:Addgene_204746 Copy
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