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CRISPR-Cas9 conjugation platform for engineering designer beta cells (RRID:SCR_026072)Copy Citation Copied
URL: https://patents.google.com/patent/US20220162649A1/en
Proper Citation: CRISPR-Cas9 conjugation platform for engineering designer beta cells (RRID:SCR_026072)
Description: This technology relies on newly described chemical modifications that allow site-specific and multiple-site conjugation of a wide assortment of molecules on both the termini and internal sites of Cas9, creating a platform for endowing Cas9 with diverse functions. Using this platform, Cas9 can be modified to more precisely incorporate exogenously supplied single-stranded oligonucleotide donor (ssODN) at the DNA break site. We demonstrate that the multiple-site conjugation of ssODN to Cas9 significantly increases the efficiency of precision genome editing, and such a platform is compatible with ssODNs of diverse lengths.
Resource Type: instrument resource
Defining Citation: PMID:32792475
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