• Register
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.


Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.


Identification of a cell protein (FIP-3) as a modulator of NF-kappaB activity and as a target of an adenovirus inhibitor of tumor necrosis factor alpha-induced apoptosis.

FIP-3 (14.7K interacting protein) was discovered during a search for cell proteins that could interact with an adenovirus protein (Ad E3-14.7K) that had been shown to prevent tumor necrosis factor (TNF)-alpha-induced cytolysis. FIP-3, which contains leucine zippers and a zinc finger domain, inhibits both basal and induced transcriptional activity of NF-kappaB and causes a late-appearing apoptosis with unique morphologic manifestations. Ad E3-14.7K can partially reverse apoptotic death induced by FIP-3. FIP-3 also was shown to bind to other cell proteins, RIP and NIK, which previously had been described as essential components of TNF-alpha-induced NF-kappaB activation. In addition, FIP-3 inhibited activation of NF-kappaB induced by TNF-alpha, the TNFR-1 receptor, RIP, NIK, and IKKbeta, as well as basal levels of endogenous NF-kappaB in 293 cells. Because the activation of NF-kappaB has been shown to inhibit apoptosis, FIP-3 appears both to activate a cell-death pathway and to inhibit an NF-kappaB-dependent survival mechanism.

Pubmed ID: 9927690


  • Li Y
  • Kang J
  • Friedman J
  • Tarassishin L
  • Ye J
  • Kovalenko A
  • Wallach D
  • Horwitz MS


Proceedings of the National Academy of Sciences of the United States of America

Publication Data

February 2, 1999

Associated Grants

  • Agency: NCI NIH HHS, Id: 5T32CA 09060
  • Agency: NCI NIH HHS, Id: CA13330
  • Agency: NCI NIH HHS, Id: R01 CA72963

Mesh Terms

  • Adenoviridae
  • Adenovirus E3 Proteins
  • Amino Acid Sequence
  • Apoptosis
  • Carrier Proteins
  • Cell Line
  • Cell Survival
  • Glutathione Transferase
  • Humans
  • I-kappa B Kinase
  • Leucine Zippers
  • Molecular Sequence Data
  • Molecular Weight
  • NF-kappa B
  • Protein Kinases
  • Protein-Serine-Threonine Kinases
  • Proteins
  • Receptor-Interacting Protein Serine-Threonine Kinases
  • Recombinant Fusion Proteins
  • Recombinant Proteins
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Transcriptional Activation
  • Tumor Necrosis Factor-alpha
  • Zinc Fingers