Expression of the human telomerase catalytic component, hTERT, in normal human somatic cells can reconstitute telomerase activity and extend their replicative lifespan. We report here that at twice the normal number of population doublings, telomerase-expressing human skin fibroblasts (BJ-hTERT) and retinal pigment epithelial cells (RPE-hTERT) retain normal growth control in response to serum deprivation, high cell density, G1 or G2 phase blockers and spindle inhibitors. In addition, we observed no cell growth in soft agar and detected no tumour formation in vivo. Thus, we find that telomerase expression in normal cells does not appear to induce changes associated with a malignant phenotype.
Pubmed ID: 9916802 RIS Download
Mesh terms: Aspartic Acid | Cell Line | Cell Line, Transformed | Cell Transformation, Neoplastic | Cyclin-Dependent Kinase Inhibitor p16 | DNA-Binding Proteins | Enzyme Inhibitors | Humans | Hydroxyurea | Nucleic Acid Synthesis Inhibitors | Phenotype | Phosphonoacetic Acid | Phosphorylation | Protein Biosynthesis | Proteins | RNA | Retinoblastoma Protein | Telomerase | Tumor Cells, Cultured
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