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Cyclin E associates with BAF155 and BRG1, components of the mammalian SWI-SNF complex, and alters the ability of BRG1 to induce growth arrest.

SWI-SNF complexes have been implicated in transcriptional regulation by chromatin remodeling. We have identified an interaction between two components of the mammalian SWI-SNF complex and cyclin E, an essential cell cycle regulatory protein required for G1/S transition. BRG1 and BAF155, mammalian homologs of yeast SWI2 and SWI3, respectively, are found in cyclin E complexes and are phosphorylated by cyclin E-associated kinase activity. In this report, we show that overexpression of BRG1 causes growth arrest and induction of senescence-associated beta-galactosidase activity, which can be overcome by cyclin E. Our results suggest that cyclin E may modulate the activity of the SWI-SNF apparatus to maintain the chromatin in a transcriptionally permissive state.

Pubmed ID: 9891079

Authors

  • Shanahan F
  • Seghezzi W
  • Parry D
  • Mahony D
  • Lees E

Journal

Molecular and cellular biology

Publication Data

February 12, 1999

Associated Grants

None

Mesh Terms

  • Amino Acid Sequence
  • Cell Cycle
  • Cell Line
  • Cell Size
  • Cyclin E
  • DNA Helicases
  • DNA-Binding Proteins
  • Fungal Proteins
  • Humans
  • Molecular Sequence Data
  • Nuclear Proteins
  • Phenotype
  • Retinoblastoma Protein
  • Saccharomyces cerevisiae Proteins
  • Trans-Activators
  • Transcription Factors
  • Transfection