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Cloning of AIP1, a novel protein that associates with the apoptosis-linked gene ALG-2 in a Ca2+-dependent reaction.

http://www.ncbi.nlm.nih.gov/pubmed/9880530

ALG-2 is a 22-kDa calcium-binding protein necessary for cell death induced by different stimuli in 3DO T-cell hybridoma. 3DO cell clones depleted of ALG-2 protein exhibit normal caspases activation, suggesting that ALG-2 function is required downstream or is independent of caspase proteases activity for apoptosis to occur. Using the yeast two-hybrid screening system, we have isolated and characterized the mouse cDNA encoding for ALG-2 interacting protein 1 (AIP1), a novel protein that interacts with ALG-2. ALG-2 and AIP1 colocalize in the cytosol and the presence of calcium is an indispensable requisite for their association. Sequence alignment shows that AIP1 is highly similar to BRO1, a yeast protein related to components of the Pkc1p-MAP kinase cascade. Overexpression of a truncated form of AIP1 protects two different cell types from death induced by trophic factors withdrawal; thus, our data indicate that AIP1 cooperates with ALG-2 in executing the calcium-dependent requirements along the cell death pathway.

Pubmed ID: 9880530 RIS Download

Mesh terms: Amino Acid Sequence | Animals | Apoptosis | Apoptosis Regulatory Proteins | Blotting, Northern | COS Cells | Calcium | Calcium-Binding Proteins | Carrier Proteins | Cell Cycle Proteins | Cloning, Molecular | Cytosol | DNA-Binding Proteins | Endosomal Sorting Complexes Required for Transport | Fungal Proteins | HeLa Cells | Humans | Mice | Molecular Sequence Data | Molecular Weight | Rabbits | Saccharomyces cerevisiae Proteins | Transcription Factors

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