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CIR, a corepressor linking the DNA binding factor CBF1 to the histone deacetylase complex.

CBF1 is a member of the CSL family of DNA binding factors, which mediate either transcriptional repression or transcriptional activation. CSL proteins play a central role in Notch signaling and in Epstein-Barr virus-induced immortalization. Notch is a transmembrane protein involved in cell-fate decisions, and the cytoplasmic domain of Notch (NotchIC) targets CBF1. The Epstein-Barr virus-immortalizing protein EBNA2 activates both cellular and viral gene expression by targeting CBF1 and mimicking NotchIC. We have examined the mechanism of CBF1-mediated repression and show that CBF1 binds to a unique corepressor, CBF1 interacting corepressor (CIR). A CIR homolog is encoded by Caenorhabditis elegans, indicating that CIR is evolutionarily conserved. Two CBF1 mutants that were unable to bind CIR did not function as repressors, suggesting that targeting of CIR to CBF1 is an important component of repression. When expressed as a Gal4 fusion protein, CIR repressed reporter gene expression. CIR binds to histone deacetylase and to SAP30 and serves as a linker between CBF1 and the histone deacetylase complex.

Pubmed ID: 9874765


  • Hsieh JJ
  • Zhou S
  • Chen L
  • Young DB
  • Hayward SD


Proceedings of the National Academy of Sciences of the United States of America

Publication Data

January 5, 1999

Associated Grants

  • Agency: NCI NIH HHS, Id: CA42245

Mesh Terms

  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • Caenorhabditis elegans
  • Cell Nucleus
  • Cercopithecus aethiops
  • Conserved Sequence
  • DNA-Binding Proteins
  • Fluorescent Antibody Technique, Indirect
  • Histone Deacetylases
  • Humans
  • Immunoglobulin J Recombination Signal Sequence-Binding Protein
  • Molecular Sequence Data
  • Nuclear Proteins
  • Protein Binding
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • Sequence Homology, Amino Acid
  • Vero Cells