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Interaction of ZPR1 with translation elongation factor-1alpha in proliferating cells.

The zinc finger protein ZPR1 is present in the cytoplasm of quiescent mammalian cells and translocates to the nucleus upon treatment with mitogens, including epidermal growth factor (EGF). Homologues of ZPR1 were identified in yeast and mammals. These ZPR1 proteins bind to eukaryotic translation elongation factor-1alpha (eEF-1alpha). Studies of mammalian cells demonstrated that EGF treatment induces the interaction of ZPR1 with eEF-1alpha and the redistribution of both proteins to the nucleus. In the yeast Saccharomyces cerevisiae, genetic analysis demonstrated that ZPR1 is an essential gene. Deletion analysis demonstrated that the NH2-terminal region of ZPR1 is required for normal growth and that the COOH-terminal region was essential for viability in S. cerevisiae. The yeast ZPR1 protein redistributes from the cytoplasm to the nucleus in response to nutrient stimulation. Disruption of the binding of ZPR1 to eEF-1alpha by mutational analysis resulted in an accumulation of cells in the G2/M phase of cell cycle and defective growth. Reconstitution of the ZPR1 interaction with eEF-1alpha restored normal growth. We conclude that ZPR1 is essential for cell viability and that its interaction with eEF-1alpha contributes to normal cellular proliferation.

Pubmed ID: 9852145 RIS Download

Mesh terms: Amino Acid Sequence | Animals | COS Cells | Carrier Proteins | Cell Cycle | Cell Division | Cell Line | Consensus Sequence | Fungal Proteins | G2 Phase | Gene Deletion | Genes, Fungal | Genotype | Humans | Mammals | Mice | Mitosis | Molecular Sequence Data | Peptide Elongation Factor 1 | Peptide Elongation Factors | Recombinant Proteins | Restriction Mapping | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Schizosaccharomyces | Sequence Alignment | Sequence Homology, Amino Acid | Zinc Fingers

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Associated grants

  • Agency: NCI NIH HHS, Id: CA 58396

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DDBJ - DNA Data Bank of Japan

The sole nucleotide sequence data bank in Asia, which is officially certified to collect nucleotide sequences from researchers and to issue the internationally recognized accession number to data submitters. It is one of 3 summit databanks that construct the DDBJ/EMBL/GenBank International Nucleotide Sequence Database, which was established through cooperative work with EBI in Europe and NCBI in USA. Since the collected data is exchanged with EMBL-Bank/EBI; European Bioinformatics Institute and GenBank/NCBI; National Center for Biotechnology Information on a daily basis, the three data banks share virtually the same data at any given time. The virtually unified database is called INSD; International Nucleotide Sequence Database. DDBJ collects sequence data mainly from Japanese researchers, but accepts data and issues accession numbers to researchers in other countries.

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