• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Three novel proteins of the syntaxin/SNAP-25 family.

Intracellular membrane traffic is thought to be regulated in part by soluble N-ethylmaleimide-sensitive factor-attachment protein receptors (SNAREs) through the formation of complexes between these proteins present on vesicle and target membranes. All known SNARE-mediated fusion events involve members of the syntaxin and vesicle-associated membrane protein families. The diversity of mammalian membrane compartments predicts the existence of a large number of different syntaxin and vesicle-associated membrane protein genes. To further investigate the spectrum of SNAREs and their roles in membrane trafficking we characterized three novel members of the syntaxin and SNAP-25 (synaptosome-associated protein of 25 kDa) subfamilies. The proteins are broadly expressed, suggesting a general role in vesicle trafficking, and localize to distinct membrane compartments. Syntaxin 8 co-localizes with markers of the endoplasmic reticulum. Syntaxin 17, a divergent member of the syntaxin family, partially overlaps with endoplasmic reticulum markers, and SNAP-29 is broadly localized on multiple membranes. SNAP-29 does not contain a predicted membrane anchor characteristic of other SNAREs. In vitro studies established that SNAP-29 is capable of binding to a broad range of syntaxins.

Pubmed ID: 9852078

Authors

  • Steegmaier M
  • Yang B
  • Yoo JS
  • Huang B
  • Shen M
  • Yu S
  • Luo Y
  • Scheller RH

Journal

The Journal of biological chemistry

Publication Data

December 18, 1998

Associated Grants

None

Mesh Terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • COS Cells
  • Cell Line
  • Databases, Factual
  • Endoplasmic Reticulum
  • Ethylmaleimide
  • Evolution, Molecular
  • Humans
  • Membrane Proteins
  • Molecular Sequence Data
  • Multigene Family
  • Nerve Tissue Proteins
  • Phylogeny
  • Qa-SNARE Proteins
  • Rats
  • Recombinant Proteins
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Synaptosomal-Associated Protein 25
  • Transfection