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Identification of a proline-binding motif regulating CD2-triggered T lymphocyte activation.

An intracellular protein termed CD2 binding protein 2 (CD2BP2), which binds to a site containing two PPPGHR segments within the cytoplasmic region of CD2, was identified. Mutagenesis and NMR analysis demonstrated that the CD2 binding region of CD2BP2 includes a 17-aa motif (GPY[orF]xxxxM[orV]xxWxxx GYF), also found in several yeast and Caenorhabditis elegans proteins of unknown function. In Jurkat T cells, over-expression of the isolated CD2BP2 domain binding to CD2 enhances the production of interleukin 2 on crosslinking of CD2 but not the T cell receptor. Hence, a proline-binding module distinct from SH3 and WW domains regulates protein-protein interactions.

Pubmed ID: 9843987

Authors

  • Nishizawa K
  • Freund C
  • Li J
  • Wagner G
  • Reinherz EL

Journal

Proceedings of the National Academy of Sciences of the United States of America

Publication Data

December 8, 1998

Associated Grants

  • Agency: NIAID NIH HHS, Id: AI21226
  • Agency: NIAID NIH HHS, Id: AI27581

Mesh Terms

  • Adaptor Proteins, Signal Transducing
  • Amino Acid Sequence
  • Animals
  • Antigens, CD2
  • Base Sequence
  • Binding Sites
  • Caenorhabditis elegans
  • Carrier Proteins
  • DNA Mutational Analysis
  • Humans
  • Lymphocyte Activation
  • Molecular Sequence Data
  • Proline
  • Saccharomyces cerevisiae
  • Sequence Analysis
  • T-Lymphocytes