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Identification of APN2, the Saccharomyces cerevisiae homolog of the major human AP endonuclease HAP1, and its role in the repair of abasic sites.

Genes & development | Oct 1, 1998

http://www.ncbi.nlm.nih.gov/pubmed/9765213

Abasic (AP) sites arise in DNA through spontaneous base loss and enzymatic removal of damaged bases. APN1 encodes the major AP-endonuclease of Saccharomyces cerevisiae. Human HAP1 (REF1) encodes the major AP endonuclease which, in addition to its role in DNA repair, functions as a redox regulatory protein. We identify APN2, the yeast homolog of HAP1 and provide evidence that Apn1 and Apn2 represent alternate pathways for repairing AP sites. The apn1Delta apn2Delta strain displays a highly elevated level of MMS-induced mutagenesis, which is dependent on the REV3, REV7, and REV1 genes. Our findings indicate that AP sites are highly cytotoxic and mutagenic in eukaryotes, and that the REV3, REV7-encoded DNA polymerase zeta mediates the mutagenic bypass of AP sites.

Pubmed ID: 9765213 RIS Download

Mesh terms: Amino Acid Sequence | Carbon-Oxygen Lyases | DNA Repair | DNA-(Apurinic or Apyrimidinic Site) Lyase | Deoxyribonuclease IV (Phage T4-Induced) | Fungal Proteins | Genes, rev | Humans | Molecular Sequence Data | Mutation | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins

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Associated grants

  • Agency: NCI NIH HHS, Id: CA41261
  • Agency: NCI NIH HHS, Id: CA53791
  • Agency: NIGMS NIH HHS, Id: GM19261

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