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The WASp homologue Las17p functions with the WIP homologue End5p/verprolin and is essential for endocytosis in yeast.

Several end mutations that block the internalisation step of endocytosis in Saccharomyces cerevisiae also affect the cortical actin cytoskeleton [1]. END5 encodes a proline-rich protein (End5p or verprolin) required for a polarised cortical actin cytoskeleton and endocytosis [2,3]. End5p interacts with actin [4], but its exact function is not yet known. To help elucidate End5p function, we sought other End5p-interacting proteins and identified the LAS17/BEE1 gene (encoding the yeast homologue of the human Wiskott-Aldrich Syndrome protein, WASp) as a high-copy-number suppressor of the temperature-sensitive growth and endocytic defects of end5-1 cells (carrying a frameshift mutation affecting the last 213 residues of End5p). LAS17 is unable to suppress a full deletion of END5 (end5 delta), however, suggesting that the defective End5-1p in end5-1 mutants may be stabilised by Las17p. The amino terminus of Las17p interacts with the carboxyl terminus of End5p in the yeast two-hybrid system and similar interactions have been shown between WASp and a mammalian End5p homologue, WASp-interacting protein (WIP) [5]. As las17 delta deletion mutants are blocked in endocytosis, we conclude that Las17p and End5p interact and are essential for endocytosis.

Pubmed ID: 9742397


  • Naqvi SN
  • Zahn R
  • Mitchell DA
  • Stevenson BJ
  • Munn AL


Current biology : CB

Publication Data

August 27, 1998

Associated Grants

  • Agency: NIGMS NIH HHS, Id: 5F32GM18002-03
  • Agency: NIGMS NIH HHS, Id: GM30027

Mesh Terms

  • Carrier Proteins
  • Cytoskeletal Proteins
  • Endocytosis
  • Frameshift Mutation
  • Fungal Proteins
  • Gene Dosage
  • Genes, Fungal
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Microfilament Proteins
  • Nerve Tissue Proteins
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Sequence Deletion
  • Suppression, Genetic
  • Temperature
  • Wiskott-Aldrich Syndrome
  • Wiskott-Aldrich Syndrome Protein
  • Wiskott-Aldrich Syndrome Protein, Neuronal