Our hosting provider will be performing UPS maintenance on Tuesday, Oct 25, 2016 between 8 AM and 5 PM PDT. SciCrunch searching services will be down during this time.

Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

alpha-Catenin-vinculin interaction functions to organize the apical junctional complex in epithelial cells.


alphaE-catenin, a cadherin-associated protein, is required for tight junction (TJ) organization, but its role is poorly understood. We transfected an alphaE-catenin-deficient colon carcinoma line with a series of alphaE-catenin mutant constructs. The results showed that the amino acid 326-509 domain of this catenin was required to organize TJs, and its COOH-terminal domain was not essential for this process. The 326-509 internal domain was found to bind vinculin. When an NH2-terminal alphaE-catenin fragment, which is by itself unable to organize the TJ, was fused with the vinculin tail, this chimeric molecule could induce TJ assembly in the alphaE-catenin-deficient cells. In vinculin-null F9 cells, their apical junctional organization was impaired, and this phenotype was rescued by reexpression of vinculin. These results indicate that the alphaE-catenin-vinculin interaction plays a role in the assembly of the apical junctional complex in epithelia.

Pubmed ID: 9700171


  • Watabe-Uchida M
  • Uchida N
  • Imamura Y
  • Nagafuchi A
  • Fujimoto K
  • Uemura T
  • Vermeulen S
  • van Roy F
  • Adamson ED
  • Takeichi M


The Journal of cell biology

Publication Data

August 10, 1998

Associated Grants


Mesh Terms

  • Binding Sites
  • Cell Communication
  • Cytoskeletal Proteins
  • Epithelial Cells
  • Humans
  • Intercellular Junctions
  • Membrane Proteins
  • Phosphoproteins
  • Recombinant Fusion Proteins
  • Tumor Cells, Cultured
  • Vinculin
  • Zonula Occludens-1 Protein
  • alpha Catenin