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A new NEDD8-ligating system for cullin-4A.

NEDD8 is a ubiquitin (Ub)-like protein. Here we report a novel ubiquitinylation-related pathway for modification by NEDD8. NEDD8 was activated by an E1 (Ub-activating enzyme)-like complex, consisting of APP-BP1 and hUba3 with high respective homologies to the amino- and carboxy-terminal regions of E1 and then linked to hUbc12 (a human homolog of yeast Ub-conjugating enzyme Ubc12p). The major target protein modified by NEDD8 was found to be Hs-cullin-4A (Cul-4A), a member of the family of human cullin/Cdc53 proteins functioning as an essential component of a multifunctional Ub-protein ligase E3 complex that has a critical role in Ub-mediated proteolysis.

Pubmed ID: 9694792


  • Osaka F
  • Kawasaki H
  • Aida N
  • Saeki M
  • Chiba T
  • Kawashima S
  • Tanaka K
  • Kato S


Genes & development

Publication Data

August 1, 1998

Associated Grants


Mesh Terms

  • Amino Acid Sequence
  • Animals
  • Cell Cycle Proteins
  • Cullin Proteins
  • DNA-Binding Proteins
  • Humans
  • In Vitro Techniques
  • Ligases
  • Molecular Sequence Data
  • Rabbits
  • Saccharomyces cerevisiae Proteins
  • Sequence Homology, Amino Acid
  • Ubiquitin-Protein Ligases
  • Ubiquitins